Ciated With Apples Carried By Entry Passengers And Detection Of Some Important Panthogens

To further improve the entry plant quarantine of fruit disease, and provide information technology support for China’s fruit disease detection, a risk analysis on diseases which could be carried by imported apples was conducted, based on comprehensive collection of data and information. Risk management measures were also proposed in this article. Isolation and culture and identification of morphology and molecular biology were carried out on the quarantine disease, Neofabraea sp. RT-Realtime detection method was established for Botryosphaeria dothidea, which seriously threaten apples. The article mainly includes:(1) An overview of data of China’s apple distribution, production, Cross-border trade, and a brief introduction about important apple diseases.(2)According to data form Nanjing Port and Chinese Academy of Science, statistics of category and quantity of fruit carried by travellers entering China and pathogens on these fruit was figured out. Totally 50 kinds of apple diseases were collected. A list of 14 pests was determined based on whether they had distribution in China and whether they had been treated by the government. Risk analysis on these 14 pests was conducted based on indicators of possibility of entry, possibility of colonization and spreading, and economic impact. The results showed that Botryosphaeria stevensii, Monilinia laxa, Monilinia fructigena, Monilinia fructicola, Neofabraea sp., Prunusnecrotic ringspot virus, Veniuria inaequalis, Sphaeropsis pyriputrescecs were highly dangerous, Erwinia bniamyces pyri, Gymnosporangium clavipes, Sphaeropsis pamylovora, Phialophora malorum, Phacidiopycnis washingtonensis were medium dangerous, Phytophthora syringae were least dangerous. Risk management measures were made to deal with dangerous pests.(3) Symptom observation, isolation and culture, morphological and molecular biological identification were carried out for Neofabraea sp. RT-Realtime detection method was established to quarantine Botryosphaeria dothidea, and was able to carry out characterized amplification of the bacteria. Amplification result was still significant even though the DNA template concentration was only 1 Opg/L.