Effects Of Deoxynivalenol In Diets On Performance Of Broiler Chickens And Its Residues In Tissues

The presence of mycotoxins in poultry feeds is a significant factor for financial losses to animal industries. Deoxynivalenol (DON) is the most common trichothecene mycotoxin detected globally in contaminated feedstuffs. As a mycotoxin, DON causes losses in livestock production and poses a health problem to livestock and humans consuming contaminated cereal products. The mode of toxic action of DON is the inhibition of protein synthesis and thus affects rapidly dividing cells, such as those of the gastrointestinal tract and immune system. This induces susceptibility to disease.In this experiment, corn was inoculated with Deoxynivalenol to obtain moldy corn with a certain toxin concentration, and the concentration of DON was detected by HPLC-MS/MS. Then diet made by the moldy corn was fed to determine vivo metabolism and distribution of Deoxynivalenol after 42 days in broilers and its effects on growth performance and skeletal biology. One hundred and twenty broiler chicks (Arbor Acres) of both sexes were collected in the present study. Chickens were randomly allocated to 4 groups, each group had 30 birds with 3 pens of 10 birds. Moldy diet was fed at 7 d of age, the concentration of DON toxin in 4 groups was 5ppm (low-dose group),8ppm (middle-dose group), 10ppm (high-dose group) and Oppm (control group). After 42 d of age, all birds were fed with normal diet. At 42 d of age, body weight, feed intake, relative liver weight, plasma Ca, P and ALP, and bone length, radiography, breaking strength of tibia and femur were determined; The metabolism and distribution of DON toxin at 0 h,6 h,12 h,24 h,48 h,72 h and 96 h since 42 d of age were detected. The results showed that after activation, culture and inoculation of Fusarium graminearum, the concentration of DON in moldy corn was 67.38 mg/kg, which was the highest among toxins (P<0.01). Feeding moldy diet with Deoxynivalenol had no significant effects on body weight, feed intake and relative liver weight. Deoxynivalenol addition significantly increased plasma P andCa level s in middle-dose group, and plasma ALP activity of high-dose group. Bone length was significantly decreased by Deoxynivalenol. Tibia breaking strength with Deoxynivalenol treatment was significantly lower than the control, while no significant differences in bone radiography and tibia ash were observed. The histological result revealed that Deoxynivalenol-contamined diets led to the shedding of intestinal epithelial cells in broilers. There was not obvious accumulation of Deoxynivalenol in the tissues and organs of birds after 42 d Deoxynivalenol treatment.