Screening Of Snp Sites And Primers Used For Pork Tracing

China holds the biggest pork production and consumption in the world, and the pork has been the main meat product in China, thus it’s necessary to develop a traceable system for pork production. Along with the development of high-throughput sequencing, the DNA markers have been used to establish traceability systems. Among the different kinds of DNA markers, the SNP (Single Nucleotide Polymorphisms) marker, as a simple and low cost genetic marker, seems to dominate on traceability field. Duroc, Landrace and Yorkshire pigs were the main commercial pig breeds. The identification of DNA in these pigs individual identity was studied, for the archives building of boars. And a new genetic marker, "DNA fragment with multigenotypes", was developed for individual identification in swine and pork tracing.I, In order to find SNPs which are available in individual identification of pigs,8 primer pairs were designed that performed well, and 63 SNPs were found. Finally the SNPs which could be used for individual identification of the three breeds in this study had heterozygosity≥0.10 and the number of SNPs genotype in each breed respectively was 131,220(Duroc),2,160(Landrace) and 6,480(Yorkshire). Theoretically this genotype strategy based on these SNPs could completely fulfill the individual identification and pork production traceability in cultivations that developed Duroc pigs.II, To gain more SNPs, a new genetic marker, "DNA fragment with multigenotypes", was developed for individual identification in swine and pork tracing. The "DNA fragment with multigenotypes" was more effective than other genetic markers in individual identification and tracing, for there were more genotypes with the more SNPs in it. In this study,76 pairs of primers were designed, of which 54 pairs gained "DNA fragment with multigenotypes" by PCR, and the rate to get "DNA fragment with multigenotypes" was close to 80%. By this mean, more DNA fragments that contain many SNPs could be gained, and markers consisted of these DNA fragments would be an effective tool for individual identification in swine and pork tracing.