| The study was conducted to investigate metabolizable energy (ME) and amino acids availability (AAA) of 30 corn samples and 30 soybean samples, and explore new bioassay for determining the bioavailability values of poultry feeds suitable for laying hens. Near-infrared Reflectance Spectroscopy (NIRS) was established based on the ME and AAA of 30 of 30 corn samples and 30 soybean samples.A completely randomized design was used and each sample of feed was a treatment with 8 replicates each and 1 laying hen in each replicate. Bioavailability values of energy and amino acids of 30 corn and 30 soybean meal were investigated by the trained feeding combined with a total collection of excreta, the results showed as follows:1. When fed corn test diet, the average feed intake and egg production was (65.20 ± 4.55) g and 5.77±0.63, the average of egg weight, albumen height, Haugh unit, egg shell strength, egg shell thickness, yolk relative weight were (51.17±2.61) g, (7.06±0.87) mm, (84.62±4.40), (3.80±0.43) kgf1, (31.14±1.88) 10-2mm, (28.15±1.05)%; The range of 30 corn ME values in laying hens were 11.37～16.91 MJ/Kg, with average value of (15.26±0.87) MJ/Kg. The range of 30 corn total AAA (TAAA), total essential AAA (TEAAA) in laying hens were 82.06%～93.23%,82.37%-92.97%, with average value of (87.47±5.95)%, (86.70±7.26)%, respectively. The average EAAA of 30 corn samples in Lys was (77.93±5.22)%.2. When fed soybean meal test diet, the average feed intake and egg production was (66.72±10.08) g and 5.57±0.68, egg weight, albumen height, Haugh unit, egg shell strength, egg shell thickness, yolk relative weight were (52.13±1.94) g, (6.87±0.86) mm, (78.91±7.59), (3.34±0.37) kgf1, (32.81±1.29) 10-2mm, (29.01±0.99)%. The range of 30 soybean ME values in laying hens were 11.95～14.87 MJ/Kg, with average value of (13.24±0.67) MJ/Kg. The range of 30 soybean total AAA(TAAA), total essential AAA (TEAAA) in laying hens were 89.99%～94.96%,91.18%-96.34%, with average value of (92.63±1.22)%, (94.10±1.23)%, respectively. The average EAAA of 30 soybean samples in Lys was (94.81±1.11)%, with the range of 92.16%～96.77%.3. R2cal, R2cv, R2val and RMSEP of FNIRS model for ME of corn were 0.99,0.94,0.95, 0.13 MJ/kg. R2cal, R2cv, R2val and RMSEP of FNIRS model for AAA of corn were 0.91～0.99,0.81～0.94,0.79～0.97,0.10%～1.66%.4. R2cal, R2cv, R2val and RMSEP of FNIRS model for ME of soybean were 0.84,0.99,0.81, 0.22 MJ/kg. R2cal, R2cv, R2vai and RMSEP of FNIRS model for AAA of soybean were 0.76～0.97,0.94～0.99,0.62～0.99,0.06%～1.00%.These results indicate as follows:1) Under the experimental conditions, with the Lohmann laying hens as bioavailability assessment model of corn and soybean meal, laying hens can maintain egg production and egg quality was normal; 2) The ME, AAA of different sources of corn and soybean meal in laying hens are different; 3) It can be established prediction model of ME and AAA of maize and soybean meal by near infrared spectroscopy technology, with high reliability and less error prediction. |
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