| It is current research focuses in animal husbandry industry that to replace antibody with traditional Chinese medicine to prevent and control animal diseases. But there are some problems during the application of the Chinese veterinary drugs: ① The drugs are mainly made of the raw materials into the form of powder, so the availabilities of the active ingredients are low, and the plays of the pharmacodynamics are slow. ② The dosage is high, generally occupy 1%-3% and even 5%-10% in the feed, because the Chinese medicine products in market are mainly crude products according to the formulation. ③ Most prescriptions are ancient, and cannot meet the new demands of the fish breeding and poultry raising industry. ④ The active ingredients of the raw materials of Chinese medicine differs a lot because of the places of production, picking seasons, and picking frequency, which results in the unstable efficiency, unspecific controllable index, different quality during the production and unavailability of testing the quality for the customers. ⑤ Some companies sell antibodies and chemicals in the name of Chinese medicine, which may result in the toxicosismedicinalis and disorder of body immune functions of animals.Wuxi Zhengda Poultry Co.,Ltdmeans to develop folk Scutellaria detoxification powder into extractive powder.The research systematically observe the extraction process,external anti-bacterial effect and the prevention and control of Piglet diarrhea and Chicken Colibacillosis.The research aims to establish the production process, testify its Pharmacological effectand provide experimental data for applying the registration of new veterinary medicine.Test 1. Research of production technology Through the research of the production process, this test successfully extract the active ingredients, so that the clinical delivery of drugs is more convenient, the absorption and utilization are more effective, the effects are better and the costs are lowered. The main ingredients of Scutellaria detoxification powder include baicalin, quercetin and oleanolicacid, which have strong inhibitory effect on bacterials of gastrointestinal diseases. On the other hand, the baicalin’s active effect is obvious, and make up over 90%in the skullcap; besides, its quality is stable, sobaicalin is taken as the main index ingredient of the active ones. Take the extraction percentage of the baicalin as the index of the extraction process, and choose the extraction method through orthogonal test. The result shows that among Soaking method, Seeping method and back-flowing method,the last method has the highest extraction percentage, so we take this method.Among the 4 factors of orthogonal test, only B fact(Volume of ethanol) is significant factor, and among its K-value, K2 is the biggest(1.763), so the ethanol should be 10 times as before. With regard to the factor A, P>0.05, and K2 is the biggest among its K values, so the concentration of the ethanol is 50%; With regard to the factor C, K1 is close to K3, so we take K3 for twice; And factor D, K1, K2 and K3 have big difference, so we take the value on 2-hour(the first time) and 1-hour(the second time). Finally we establish the best extraction process as following:having the 10 times amoung of 50% ethanol back-flow for 2 hours.The first time,have the 6 times amount ethanol back-flow for 2 hours, and the second time, have the 4 times amount ethanol back-flow for 1 hour. The quality can meet the demand in these 3 amounts, and the transportation rate of baicalin can reach 72%.Test 2.The bacteriostatic test of scutellaria detoxification powder The test measures the minimum inhibitory concentration of Scutellaria Detoxification Powder on O2, O18 and O78 e. coli. The result shows that the diameter of the bacteriostatic circle of O2-e.coli formed by Scutellaria Detoxification Powder is 13.3+2.5 mm, and for O18-e.coli, it’s 7.7+0.6 mm, and O78 is 10.7+0.6 mm. All of them are medium sensitive. Through the method of test-tube duple dilution, we test that the minimum inhibitory concentration (MIC) on 02 by Scutellaria Detoxification Powder is 160 mg/ml, and the MIC on O18 and O78 is 80 mg/ml, which shows that Scutellaria Detoxification Powder has apparent bacteriostatic effect on O2, O18 and O78-e.coli.Test 3. The treatment test of chicken colibacillosis with scutellaria detoxification powder 350 50-days-old Roman roosters are randomly divided into 7 groups. The blank control group was feed isolated and did nothing to them. The rest 6 groups were given colibacillus and put into different conditions.3 groups are experimental groups with 0.1%, 0.05% and 0.025% of Scutellaria detoxification powder. The control group was constantly given the feed with 0.02% florfenicol. Positive control group without any drug. Observe the clinical symptoms of each group, and record the number of the death, alolaxata, depressed chickens and the growth. Measure the effects after 10-day. The result shows that the group of high dosage and medium dosage has higheroverall efficiency and lower death rate, higher average weight and weight increase, lower number of chickens with also laxata and depression. The conclusion is that scutellaria detoxification powder can effectively be used to prevent the chicken colibacillosis, and the recommended dosage is 0.05% added in the feed for constant 5 days.Test 4. The prevention test of chicken colibacillosis with scutellaria detoxification powder 350 45-day-old Roman roosters are divided into 7 groups. The blank control group was feed isolated and did nothing to them.3 groups are experimental groups with 0.05%,0.025% and 0.0125% of scutellaria detoxification powder. The control group was given the feed with 0.01% florfenicol. Positive control group without any drug. Observe the clinical symptoms of each group, and record the number of the death, alolaxata, depressed chickens and the growth. Measure the effects after 10-day. The result shows that the group of high dosage and medium dosage have higheroverall efficiency and lower death rate, higher average weight and weight increases, lower number of chickens with alolaxata and depression. The conclusion is that scutellaria detoxification powder can effectively be used to prevent the chicken colibacillosis, and the recommended dosage is 0.025% added in the feed for constant 5 days. |
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