The Locatng Analysis Of The Transcriptional Factor MoMsn2 And The Function Analysis Of Its Related Genes In Magnaporthe Oryzae

Rice blast, the important global disease, is one of the most serious rice diseases in north and south rice area of China. We loss of hundreds of millions of rice every year.According to the National Agricultural Technology Extension and Service Center forecast, rice blast occurred or above in susceptible strains in southwest, northeast and southern, the areas occurred over 5.3 million hm2.So, to Strengthen research rice blast has significance of controlling prevalent harm. Now we have monitored and forecasted to prevalence of rice blast, and establish the prevalence of risk assessment system. But the methods of Monitoring and forecasting have a lot of problems and technical difficulties. Rice blat is occurred by Magnaporthe oryzae, but the pathogens Pathogenicity is easy to change. Even though we try to find the reason to the possible ways to variation, we just understand the growth and development as well as the molecular mechanism of pathogenicity to control the plant disease. In recent years, With the establishment of rice blast fungus published genome sequence and genetic transformation of the operating system, it allows us to accelerate footsteps to study in the pathogenic mechanisms of gene function and growth. At the same time, it provide theoretical support for us to find the new targets of fungus fungicides. In eukaryotic cells, MAPK signaling pathway, important cell-mediated responses, exists in a variety of organisms （including yeast and mammalian cells）. MAPK signaling pathway involves in cell growth, development, division, cell death and functional synchronization between processes and other physiological reactions. In Saccharomyces cerevisiae, MAPK pathway builds up with Fus3, Kss1, Slt2, Hog1, and Smk1. These signaling pathways are received different extracellular stimuli and regulate various biological processes. Recent studies show, Hogl pathway widely exists in filamentous fungi, and are closely related to stress response regulator of fungi.The object of this study is MoMsn2,which interact with MoOsm1. MoMsn2 exists in Hogl signaling pathway. It is a class of encoding zinc finger protein transcription factor, similar with ScMsn2 or ScMsn4. It can cause the amount of expression of genes related to condia drops. We can find aerial hyphae reduced and hardly produce conidia when we destruct the gene. The condia of The MoMSN2 mutant are abnormal and melanin formation of defects, at the same time, The MoMSN2 mutant can not invade host and loss of virulence. We also find that MoMsn2. MoMsn2 involved in regulating the growth of infection hyphae and extracellular laccase, peroxidase activity, sensitivity to different stress factors In the environment.We did subcellular localization, and find that MoMSN2 located in the nucleus. We did the group level analysis of gene transcription at the basis of the known MoMSN2 gene function. We get the 1254 up-regulated genes and 322 down-regulated genes.It has 888 genes with predicted binding sites in the 1256 up-regulated genes. It has 226 genes with predicted binding sites. The datas show that MoMsn2 regulates a large number of genes about the formation of spores, infection, cell wall. We find the datas have a lot of genes about the formation of spores, infection, cell wall which haved been reported. They are subject to varying degrees of expression up or down which Confirms the function of genes MoMSN2. At the same time, to further understand the mechanism of transcription of genes MoMSN2, we choose some genes to research which may have the predicted binding sites. Though the research, we find MGG₀2775 cannot produce the conidia and MGG₁0482,MGG 10105,MGG 09134,MGG 01351.MGG 01557 become more sensitive to external stress.