Analysis Of Candidate Genes Polymorphism Associated With Semen Quality And Metal Ion Concentrations In Seminal Plasma In Zie Geese

To search for mutations related to semen quality traits in Zie geese(Anser cygnoides L.), the Matrix-Assisted Laser Desorption-Ionization Time-Of-Flight Mass spectrometry (MALDI-TOF MS) technology was used in this experiment.11 SNPs from 5 candidate genes(PRL gene, FSH beta subunit gene, PRLR gene, GnRH gene, ESR gene) in 143 Zie geese were genotyped. The correlation of SNP loci and semen quality traits were analyzed.Two mutations were discovered in this test, namely the mutation A/G in PRL gene intron2 and the mutation C126T in FSH beta subunit gene intron2. The two genotypes found in PRL gene AA, AB, which genotype frequencies were 0.98,0.02, while AB phenotype had poor semen quality, which has too tiny semen volume to analysis.In FSH beta subunit gene intron2, three genotypes CC, CT, TT were found, which genotype frequencies were 0.08,0.44,0.48. The geese with TT genotype had a bigger volume than that of CC and CT. CC and TT genotype had a greater sperm density than that of CT (P<0.01), respectively. TT and CT phenotype possessed higher sperm motility than that of CC phenotype positively (P<0.01).In addition, Flame Atomic Absorption Spectrometry (FAAS) method was applied to measure the metal ion concentration in seminal plasma from Zie geese in different SQF groups. The results indicated that Zn, Ca and Cu concentrations in seminal plasma were in the order Ca>Zn>Cu. There were positive correlations between the concentration of Ca with semen motility (0.47, P<0.05) and live morphologically undamaged sperm number (0.32, P<0.05). Cu and Zn concentrations were not correlated with any semen analysis parameters.