Analysis Of Biogas Slurry Active Components And Terpenoid Transform Mechanisms

Biogas slurry is liquid residue of human and animal feces and all kinds of crop straw after the anaerobic fermentation. With the further promotion of biogas engineering in our country, as the main by product of biogas anaerobic fermentation--the amount of biogas slurry has risen sharply, which contain a variety of crop nutrient essential elements. The present study showed that biogas slurry is a kind of effective liquid fertilizer, mainly used as feed additives, leaf area fertilizer,seed soaking, etc. For the study of biogas slurry is limited to its physical and chemical properties, include mineral content, heavy metal and amino acid and B-vitamins.The process of methane produced, as the same time it’s a biochemical process involved microbial fermentation, which can convert feedlot wastes into some secondary metabolites, such as abscisic acid, gibberellin, estradiol. Furthermore, microorganism survival in the anaerobic and extreme environment often produces some novel compounds, which could be stored in the biogas slurry-the by product of anaerobic fermentation, and as a result, biogas slurries usually exhibit special properties. For example, the chicken manure biogas slurry can inhibit the growth of microorganisms,reduce plant diseases, and increase production of crops.Research has show that spraying biogas slurry on the crop has certain bacteriostasis effect, biogas slurry can prevention and cure nearly 30 kinds of crop disease, biogas slurry have effective control function for 19 kinds of insect-pests. But the mechanism of prevent diseases and insect pests in agricultural applications is unclear. However, there is no literature concerning the chemical composition of chicken manure biogas slurry up to today. In order to analysis of biogas slurry chemical composition, antibacterial mechanism and microbial transformation mechanism. Through extraction, silica gel column and lots of chromatographic methods for separation of biogas slurry from chicken manure. The research was conducted for the analysis of component of chicken fece biogas slurry.Three compounds was extracted and prufied from biogas slurry by silica gel column and lots of chromatographic methods. Then the cytotoxicity of three kinds of compounds wasevaluated. And the microbial transformation of Soyasapogenol B has carried on, in order to explore the origin of pentacyclic triterpenoids in biogas slurry.We obtained three compounds in this research. Structure was identification by mass spectrometry(MS) and nuclear magnetic resonance(NMR) method and the literature comparative. The three compounds were lutein, 6-hydroxyl-3,4-dihydroquinoline-2-one, Soyasapogenol B, respectively. These compounds were evaluated for the vitro cytotoxicity. The selected cells are Hep G2、DU 145、HCT 116、NCI-H460,K562 tumor cell lines. Soyasapogenol B showed weak cytotxic against tumor cell lines NCI- H460, DU 145, IC50 values were 152.95 μg/m L and 133.65 μg/m L, respectively. All the rest of the compounds didn’t show the cytotoxic activity. One of three compounds is soyasapogenol B, which is a kind of pentacyclic triterpenoids.Our laboratory have extracted four new pentacyclic triterpenoid compounds were similar to soyasapogenol B, And the chicken feed contains soyasapogenol B, so that these compounds may be the transformed from soyasapogenol B, may be transformed in chicken intestines or biogas slurry.To explore its transformation mechanism, this study selected 5 bacterias and 11 molds, and screened strains can eventually transform soyasapogenol B. Luckily, Penicillium chrysogenum CMCC 3.3890 can transform it.The ethyl acetate extraction of fermented liquid were detected by LC-MS/MS. In the mass spectrogram showed three main parent ion peaks. Through database match, the corresponding compounds may be 3?-hydroxyl-24-Norolean-12-diene-22-one,Stigmasta-4,24-diene-3,6-dione,(9xi,10 xi,13xi,17 xi,22E,24xi)-Stigmasta-4, 6, 8(14),22-tetraen-3-one,(22E,24xi)-Stigmasta-4,6,8,22-tetraen-3-one,(4α,5α)-4-Methylergost a-8,24-Diene-3,11-dione,(17xi,22 E,24xi)-3-Hydroxystigmasta-5,8,22-trien-7-one.Proved the possible that the microbial transformation of soyasapogenol B generating new pentacyclic triterpenoid.Through the above research, it is necessary to accelerate research on the comprehensive utilization of biogas slurry. Not only can find some conversion function of microorganism and some novel compounds, but also make efficient use of biogas slurry. Then solve the problem of emissions of the biogas slurry, and protect environment. It also provides a new way for the compound conversion and synthesis.