The Establishment Of Tree Peony Grafting System In Vitro

Paeonia suffruticosa are Paeoniaceae. Paeonia L. It is also called flower of riches and honour, wood peony, ardisia crispa etc. The original country of tree peony is in china, Known as the "national beauty and heavenly fragrance peony", "the king of flowers", as the symbol of wealth and good fortune, flourishing. In recent years, useing of tissue culture for rapid propagation of peony has become a new research direction. Therefore, scholars have made a large number of related research. Research so far is mainly focus on cultivation, propagation techniques and organizational aspects of culture in China. In foreign countries, most of the species remain in the type of physiological factors and contents of research. One of the effective ways to improved varieties is using in vitro micrografting technology. But at present, there is no report about effect to tube micrografting of Paeonia suffruticosa.In this experiment, we used the induced root of the peony "Fengdan white" seeds as the test material, scion choosing varieties of Peony sub as tested materials to estab –lish sterile system, and the established sterile implant system to research with grafting method, cultivation conditions, deal with stock and scion and etc. And results were as the following:In the culture of scion, appropriate formulation of adventitious buds is MS+6-BA 2.0 mg/L+NAA0.1 mg/L, and for the buds is MS+6-BA0.5 mg/L+NAA0.1 mg/L, the appropriate illumination time is 16 h/d.In the culture of stock, the most suitable medium for embryo growth is MS+ 6-BA0.2 mg/L+IBA1.0 mg/L; the best medium for callus differentiation is MS+6-BA 1.0 mg/L+2,4-D0.5 mg/L+GA1.0 mg/L; the best medium for rooting induction is 1/2MS+IBA0.1 mg/L+NAA0.1 mg/L; the best medium for root thickening is 1/2MS +IBA 0.1 mg/L+6-BA1.0 mg/L.Using cleft grafting and top grafting method, and the result sign: the cleft grafting methodˊs survival rate is 73.33%, but the top grafting methodˊs surviva rate is 34.33%. Cleft grafting method is the best.Put different density hormone in MS medium, such as IBA, 6-BA, NAA, the result showed: 6-BA is advantage to plantlets.Cultivate the plantlet in dark for 0 d, 5 d, 10 d and 15 d. We can find that:the dark culture time is 15 days, the survival rate of grafted seeding was 0%.However dark culture for 0 d, 5 d had higher suvival rate. Dark culture for 5 d, the elongation or new leaves exposed, but 0 d, needs to be about 15 d. The result sign:dark for 15 d, survival rate is 0%, but dark for 0 d and 5 d is advantage to plantlets.In a certain range, the survival rate of tube seedlings is positively related to the concentration of sucrose, too high sucrose concentration will inhibit the survival of grafted seedlings. 30 g/L is advantage to plantlets with survival rate to be 73.33%; The survival rate of test tube seedlings increased with the increaseof sucrose concen-tration, then decreased. Therefore, 30 g/L is best.Put the plant in 20 ℃、23 ℃、26 ℃ and 29 ℃. The result sign: 23 ℃ is the optimum temperature with survival rate to be 73.33%.Agar concentration if low, medium is soft, not easy to solidification; instead, the hardness is too big. The result sign: agar concentration of 0 g /L and 3.5 g/L, is disadvantage to plantlets, but 7.0 g/L is advantage to plantlets with survival rate to be 73.33%.Rootstock and scion length also has a certain influence on the survival rate. The result sign: Root stock suitable length is 1.5 cm; Scion is 1.0 cm.The results of observation peony grafting showed, the adhesion of callus in inci-sion on is obvious.The existing varieties have been unable to meet the market requirements of varieties. In this experiment, by using the technique of tissue culture, develop suitable grafting seedlings, to provide a theoretical basis for the realization of rapid propaga-tion, cultivation of peony tube grafting techniques and new varieties.