Research On Efficient Transformation System Of Eggplant Mediated By Agrobacterium

Eggplant(solanum melongna L.), annual herb plants, an important economic vegetables in China. However, the yield and quality of eggplant is vulnerable to diseases, insects or other adverse environment. Therefore, breeding new eggplant varieties resistance to insects, diseases has great significance. Regular breeding methods and practice of eggplant play an important role in improving the new species,at the same time there are some problems, like long breeding period, farsource hybrid incompatibility and lack of germplasm resources. It is vital for us to utilize transgenic technology to cultivate new eggplant.Using eggplant cotyledons as explants, different concentrations and combinations of hormones NAA, ZT, 6-BA effects on callus induction were studied. The results showed that the ‘Baicuo’ eggplant cotyledons could induce callus, partial callus can differentiate into adventitious bud, but induction rate was significant difference. The best inducing medium for ‘Baicuo’ eggplant callus was MS+NAA 0.1mg/L+ZT 3.0mg/L and the callus rate was 97%. Best medium for inducing buds was MS+NAA 0.1mg/L+ZT 4.0mg/L+6-BA 1.5mg/L+Ag NO3 8.0mg/L and the budding rate was 82%. Ag NO3 has positive effect on bud differentiation. The explants callus induction rate and bud differentiation rate are quite different among different genetypes. Best medium for rooting is 1/2MS and the rooting rate was 80%. ‘Baicuo’ regeneration system has been successfully established.This study also carried on preliminary exploration about agrobacterium-mediated transformation of eggplant, screening hygromycin concentration which inhibit non-transformed explant,ticarcillin concentration which inhibit the growth of agrobacterium. Besides, our research also studied the influences on explants transformation caused by pre-culture time, concentration of bacterium and infection time. The results showed that 10mg/L Hygromycin concentrations can effectively select resistance shoots; 150mg/L Timentin can effectively inhibit the infection of Agribacterium; the best time of pre-culture was 2 days, the transformation rate can reach 78%; the best time of infection is 10 minutes and the transformation rate was 77%. As GUS staining results and molecular analysis show that SmCRC genes have already transformed to ‘Baicuo’ genomes. As a result, we harvested 8 transformed plants.