| Uvaria microcarpa plants belong to Annonaceae, are mainly distributed in Guangxi,Guangdong and Taiwan of China, Vietnam and Laos. There are a variety of Medicinal Uvaria genus plants can be used to cure rheumatism, traumatic injuries, and relieve swelling and pain and so on. Uvaria microcarpa plants mainly contain Annonaceous acetogenins, Polyoxygenated cyclohexenes, flavonoid and other ingredients. Jolad[1]and others used active test tracking method to separate and purify ethanol extract of roots of Uvaria accuminata to get Uvaricin, which had good antitumor activity. And Annonaceous acetogenins show cytotoxicities against most of tumor cells, as candidate molecules of cancer drugs, have broad research prospects with deepening study.Endophytic fungi is a fungus that lives within a plant for at least part of its life without causing apparent disease. According to reports, in the "endophyte- plant" is a stable symbiotic system, endophytic fungi can produce complex structure of secondary metabolites with a variety of biological activity of anti-tumor, anti-bacterial, anti-oxidation,and has become a new drug development the material source. This paper gets 13 endophytic strains from the medicinal plant Uvaria microcarpa which packed in dinghu mountain and luofu mountain. Through screening the compound abundance of crude extracts, anti-tumor activity, antioxidant activity and Tyrosinase inhibitory activity test,A092 was believed to have abundant compounds and relately good anti-tumor, antioxidant activity. Identification of A092 showed that it belonged to Arthrinium sp. And the literature shows that, due to its adaptation to the environment, different environmental sources of Arthrinium sp may change the way of metabolism and diversity structural secondary metabolite, and most of them have a strong biological activity, but there were no reportedstudies of active metabolites of endophytic fungal origin Arthrinium sp. In this paper, it is the first time to study on Arthrinium sp which is isolated from medicinal plant Uvaria microcarpa, in order to obtain compounds with biological activity from its metabolites.Using Positive phase silica gel column chromatography, Sephadex LH-20, C18 reverse phase, HPLC, preparative thin layer chromatography, recrystallization and other methods to separate and purify from the culture and the mycelia, get thirty compounds, and twenty-one of them have identified by the spectral data analysis and contrast samples, they are:flemingipanic acid(1),(-)-gynuraone(2), 2-(4-methoxy-phenyl)-ethanol(3),2-hexyl-methymaleic anhydride(4), {1-[(12E, 16E)]-12, 16-eicosadienoyl]-2-[(E, E)-7,11-octadecadienoyl]-3-stearoylglycerol}(5), mycoediketoperazine(6), libertellenone C(7),4-hydroxymethyl-4, 6-octadiene-2, 3-diol(8), dimethyl phthalate(9),di-(2-ethyl)-hexylphthalate(10), 4-hydroxyphenethyl-2-(4-hydroxyphenyl) acetate(11),4-hydroxy-17R-methylincisterol(J1), 3-methoxy-4-hydroxy benzoic acid(J2),decarboxycitrinone(J3), dibutyl phthalate(J5), flemingipanic acid(J6), 3-methyl indole(J7), p-hydroxybenzoic acid(J9), ergosterol peroxide(J10)、4-hydroxybenzal-dehyde(J11).Compounds 1, 3~5, 8~10, J1-J2, J4~J11 are isolated from the genus Arthrinium for the first time.Taking staphylococcus aureus、escherichiacoli as the tested strain, by the resazurin method to study the antimicrobial acitivity of compounds in vitro, the results show that compounds 7, 8, and J2 have some antibacterial activity. Taking SF-268, MCF-7,NCI-H460, HePG-2 as the selected cell lines, by using the SRB dyeing to conduct in vitro cytotoxin activeness research to the compounds, the results show that compounds 7, 8, and J2 have cytotoxic activities against cell line SF-268, MCF-7, HePG-2. By using the clearance rate of DPPH freedom radicals evaluate the antioxidant activity of compounds. |
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