The Study Of Virus Detection And Isolated Culture On Lanceleaf Lily Bulb

Lanceleaf Lily Thunb (Lilium lancifolium) is a kind of edible lily, holds a great demand in the market, but as a result of breeding cycle is long, and they are vulnerable to be damaged from viruses and quasi-virus, make its yield and quality can’t meet the needs of the market.Lanceleaf Lily Thunb will serve as materials in the study, we will take these methods to detect virus from the material, for example:the field observation and the serological detection and molecular biological detection; and an progenitive system of effective and rapid will be established through tissue culture technology. The main research contents and results are as follows:1Based on the field investigation, found that Lanceleaf Lily Thunb is common infected by virus in hunan province, and show mainly include about these different symptoms:dwarf plants、leaf blade short、leaf blade stagger and deformity、rosette dwarf and so on. The short leaves and leaf staggered formation rate is highest, reaching51%and48%, the other is relatively small.2The result of DAS-ELISA test shows that Lily is mainly infected by two types of virus in Hunan provinve:Cucumber mosaic virus and Lily symptomless virus, their rate of infection was75%and58%, and there are two kinds of virus infection phenomenon. The single RT-PCR and double RT-PCR two methods can be taked, to identify the virus under natural conditions, and to establish a method for simultaneous detection of multiple virus.3The establishment of tissue culture system of Lanceleaf Lily Thunb. MS as lily tissue culture the basic culture medium, additional different types and concentrations of plant growth regulator. The best induction culture medium of squama and stem section was MS++0.6mg/L6-BA+0.3mg/L NAA; MS+0.8mg/L6-BA+0.4mg/L NAA is the best for multiplication induction; The best induction culture medium for roots is1/2MS+0.2mg/L NAA。4The systemica study of three methods that can eliminate the viruses of CMV and LSV, they are stem point culture; stem point culture combined with heat treatment;stem point culture combined with ribavirin. On three methods be compared and analyzed, found the result that stem tip culture combined with heat treatment method of detoxication rate is the highest, and it also can ensure the high survival rate,...