| Objective:the objective of the research is to analyze the arable layer of different producing areas of Lonicera japonica and their physicochemical properties and to study the pertinence between those properties and the active constituents of Lonicera japonica. And with the results, it aims to improve the quality and to normalize the cultivation of Lonicera japonica and to provide scientific foundation and references for the construction of GAP basesMethodology:According to the research approaches of traditional Chinese medicine and botanical geography,the thesis will apply the research methods of modern Chinese medicine, soil analysis and phytology. Based on the investigation of the current situation of the domestic production of Lonicera japonica, the producing area is divided into several regions, considering the germplasm, planting scale, geographical location, geomorphic condition, agrotype, and climate. The soil of the arable layer and the flower bud during the same flowering phase are sampled from each regions. The study applies the national regulations of the soil standard to examine the physicochemical index of the arable layer of different producing areas of Lonicera japonica, employs high performance liquid chromatography to measure the content of the active constituent of Lonicera japonica, and uses the statistic software "SPSS 19.0" to analyze the pertinence between the content of the active constituent and the physicochemical properties.Results:physicochemical properties of different sampled regions, including the potential of hydrogen of the arable layer, nutrient element, microelement, and heavy metal element, are examined. Active constituents, chlorogenic acid and Galuteolin, are measured. The content of elements of the arable layer has a great influence on the active constituent of Lonicera japonica:the correlation coefficient of new chlorogenic acid and rapidly available potassium is 0.264, fitted equation:Y=0.264XavK; the correlation coefficient of chlorogenic acid, available potassiumand mercury is 0.281、-0.271, fitted equation:Y= 0.284Xavailable potassium-0.235 Xmercury;the correlation coefficient of cryptochlorogenic acid, ph., rapidly available potassium and available iron is 0.333、0.333、-0.337, fitted equation:Y=0.039 XPH+0.308 XeX Ca+0.161 XavK—0.257 XavFe,The coefficients of correlation of isochlorogenic acid A with soil ph value, manganese and high metal hg are -0.272,0.217 and 0.232 respectively as represented by the fitting equation of Y=-0.477 XPH+0.376Xra k-0.232 XavZn+0.199 Xhigh metal arsenic. The coefficients of correlation of isochlorogenic acid B with rapidly available potassium and high metal arsenic are 0.253 and 0.246 respectively as represented by the fitting equation of Y=0.466 XraK-0.389Xavaiiable arsenic +0.257 Xhigh metal arsenic. The coefficients of correlation of isochlorogenic acid C and rapidly available potassium are 0.216 respectively as represented by the fitting equation of Y=0.216XavK. The coefficient of correlation of galuteolin with rapidly available potassium is 0.408 as represented by the fitting equation of Y=0.387+0.284XavK+0.656X2aVK-0.115 X3avK.The coefficients of correlation of rutin with exchangeable magnesium, high metal arsenic and high metal hg are 0.243,-0.251 and -0.256 respectively as represented by the fitting equation of Y=0.216 XeXMg-0.183 Xfull metal As-0.137 Xfullmetal Hg.Conclusion:the effects of the arable layer on the active constituent of Lonicera japonica provides references for site selection of GAP cultivation bases of Lonicera japonica, and draws up guidelines for improving the quality of medical products and the management of the cultivation of Lonicera japonica. |
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