| There is a natural hydrophobic barrier-cuticle, existing on the surfaces of most of the aboveground part in plant. It is synthesized and secreted by epidermal cells, including cutin and wax. It can weaken injury caused by environmental factors and non-somatic moisture loss. With the aggravation of greenhouse effect, crop will face more and more serious drought stress, but there are not many reports on the molecular mechanism of the function of cuticle wax in adaption to drought stress.Abscisic acid, synthesized by the plant itself, is a hormone with sesquiterpene structure. Recently, many researchers have reported that ABA, as a main stress induced factor, plays a more and more important role in plants’ adaption to drought and cold. NCED is a key speed-limit enzyme regulating the synthesis of ABA. AtNCED3 is a member of the gene family that regulates the synthesis of NCED.My research selected zhonghua 10 rice and OE11-3 as experimental materials. By scanning leaf under electron microscope, measuring leaf water loss, chlorophyll extrvasation rate and leaf wax content, we found that compared with WT, OE11-3 has a higher leaf water loss rate and chlorophyll extrvasation rate, but a lower cuticle wax content. The result indicates us that the plant may adapt to a higher ABA content from itself by a slower growing speed to keeping more energy for a basic metabolism. We found that AtNCEDS expressed constitutively in rice can decrease leaf area, the number of wax mastoids, wax crystalloid on silicon cells, the mechanism need be verified further. We also came to a contrary conclusion, the rice was more stuggy in a higher exogenous ABA environment. In ripe time, leaf wax was higher than WT.In this study, we detected that there is significant difference between WT and OE11-3.It may be that the two conditions had a different effect on synthesis of cutin, supplying a base for further research on how genes regulate the synthesis of cutin. |
