Preliminary Mapping Of Cloned Plant Cuticle Genes In The Genomes Of Rice (Oryza Sativa L.) And Barley (Hordeum Spontaneum)

Plant surfaces above-ground are sealed by a multifunctional cuticle which exposed to the environment. It contains cutin and wax. The plant cuticle restricts non-stomatal water loss, in addition, the cuticle plays a important role in air exchange, reducing the threat of UV radiation, pest and disease, hindering the deposition. The structure and chemical composition are intricate, which indicate that many genes have taken part in the development and transport of plant cuticle. Numerous cuticle genes have been cloned in Arabidopsis, maize, rice and several other plants.These cloned cuticle genes were generalized in this research. They were mapped in silico on different chromosomes of rice and Arabidopsis. The analysis of homology and synteny were also conducted.We developed 12 pairs of primers based on the CAPS. Multipoint version 2.2 (Institute of Evolution, Haifa University) was used to assign the orders of 12 homologous sequences of plant cuticle genes in the barley genome.We did the preliminary mapping and analysis of cloned plant cuticle genes in the genomes of barley (Hordeum spontaneum) and rice (Oryza sativa L.). The consensus maps of these genes will play a important role in the further selection of candidate genes in a positional cloning of an unknown cuticle gene in plants.