Screening Efficient Components Of Chinese Medicine With Antiviral Activities Against Porcine Reproductive And Respiratory Syndrome Virus (PRRSV) And Investigating Their Antiviral Mechanisms

Objective:Porcine reproductive and respiratory syndrome (porcine reproductive and respiration syndrome, PRRS), also known as blue-ear disease, is a strong common infectious swine virus which has easy variation characteristics, will supplies after the outbreak PRRS caused great economic losses. Current means of prevention for the disease mainly vaccination can play a role, but there is no effective treatment for PRRS. The test selected extracts from the common anti-viral herbs honeysuckle, Radix and Epimedium natural compound chlorogenic acid, epigoitrin and icariin to Marc-145 cells in vitro model to study whether they have in vitro anti-pig (porcine reproductive and respiration syndrome virus, PRRSV) effect, is able to effectively inhibit apoptosis induced by PRRSV, and to explore the mechanism of these anti-viral medicine major component reproductive and respiratory syndrome virus.Methods:(1) by using CPE method (cytopathologic effect, CPE) and MTT (3-(4,5-dimethyithiazol-2-y1)-2,5-diphenyltetrazolium bromide, MTT) to determine the medicine extract substances maximum safe concentration (maximum no-cytotoxic concentration, MNTC) in vitro model Marc-145 cells.(2) using RT-PCR method for determining the role of PRRSV infection on Marc-145 cells was observed on Marc-145 cells PRRSV infection site by immunohistochemical methods and research of traditional Chinese medicine compound in its safe concentration of the virus invasion inhibitory effect of Marc-145 stained cells.(3) researching on Chinese security compound in its concentration on PRRSV-induced apoptosis effects of the use of the role of apoptosis detection methods.Results:Herbal extract natural compounds chlorogenic acid, epigoitrin and icariin have anti-PRRSV effect, and chlorogenic acid is most obvious. The maximum safe concentration of chlorogenic acid is:500μg/mL, epigoitrin maximum safe concentration is:1000μg/mL, the maximum safe concentration of icariin is:31.25μg/mL. Add chlorogenic acid and tables at the maximum safe concentration reported in accordance with the spring, you can make Marc-145 cells against PRRSV infection, while the number of apoptotic cells was significantly lower than that in the control group added virus drugs, and icariin PRRSV infection cannot be completely prevented Marc-145 cells, can reduce the number of virus-infected cells and play a role in the inhibition of apoptosis.Conclusion:The natural compound chlorogenic acid extract of Chinese medicine, epigoitrin has a strong effect in vitro anti-PRRSV, and PRRSV icariin vitro effect is not ideal, suggesting that chlorogenic acid and epigoitrin of antiviral mechanism may be directly inactivated PRRSV and inhibition of apoptosis induced by PRRSV to achieve, and icariin can be a certain degree of inhibition of apoptosis caused by PRRSV.