Immunoregulation Effects Of UBC13 On C57 Mice And SLE Model Mice

Object:UBC13 protein were used to intervene C57 mice and murine systemic lupus erythematosus(SLE) model, then we investigate the immune intervention effect and the possible molecular mechanism of UBC13 protein by means of immunology, serology, viscera tissue morphology and so on.Methods:C57BL/6J female mice and murine SLE model female mice (F2) aged 12-13weeks were randomly divided into blank group, PBS group and UBC13 group. UBC13 group were treated (i.p.) with UBC13 protein 25mg/Kg for 10 consecutive days; PBS group at the same time were given the same dose of PBS buffer; while Blank group were not injected anything. Serum was extracted through eyeball veins from each mouse in each group at the 0,5 and 10d. Analyze the expression of IL-10, IL-17, anti-dsDNA antibody, ANA content, the percentage of Tregs, viscera morphology, bodyweight and viscera indexes change of the six groups.Results:The results of experiment on C57BL/6J and murine SLE model mice showed that the treatments with UBC13 could significantly cause the spleen swelling and increase viscera indexes while the bodyweight indexes, ANA and IL-10 level of mice in serum were impregnability (P>0.05), viscera indexes, IL-17 and anti-dsDNA level in serum and the percentage of Tregs in spleen in UBC13 groups were significantly higher than that in PBS group and Blank group (P<0.05); From what had been observed on kidney tissue HE staining, we could safely drew a conclusion that lesions out of sync appeared in murine SLE model mice because glomerular mesangial cells increased inhomogenous; it also showed that severe infiltration of lymphocytes, eosinophilic neutrophils and other inflammatory typical changes of the UBC13 group; Compared the three groups, liver pathological changes of both the PBS group and the Blank group were lighter than the UBC13 group; it appeared different degree infiltration of inflammatory cells in UBC13 group; the UBC13 group was found extramedullary hematopoiesis, eosinophilic neutrophils and fibrin deposition in liver.Conclusions:UBC13 cause the abnormal increase of IL-17 and dsDNA antibody, but it can improve the percentage of Tregs. Further studies are needed to ascertain the dose and possible molecular mechanism of UBC13 protein and these findings are promising to provide a potential therapeutic target for autoimmune disease.