Morphological Observation On The Pancreas’ Development Of Post Embryonic Wuzhishan Pigs

The pancreas is an important glands with two functions of exocrine and ndocrine. Currently, the islet transplantation had become the ideal means of insulin-dependent diabetes treatment, and the porcine islets was considered to be the appropriate donor source. In this experiment, I took 1 day age,10 days age,40 days age,4 months age and 6 months age Wuzhishan pigs as test material. I took gross anatomy, histology and immuneehistochemical technique to study the development law systematically from the perspective of morphology. The results were as follows:1.The 1 day age,10 days age,40 days age,4 months age and 6 months age Wuzhishan pigs’pancreas could be devided into the pancreatic body,the left lobe (spleen leaf),the right lobe(duodenum leaf),the connection and the connecting bridge. They were connected to form the pancreatic ring.Post-embryonic development in the process of its weight and weight ratio increased with the increasing of day age.2.The Wuzhishan pig pancreas’ islet A,B and D cells of immunohistochemical staining results showed that the positive signal area was increasing. The 1 day age,10 day age,40 days of age,4 months age and 6 months age of Wuzhishan pigs’ pancreas insulin immune reaction cell distribution area were 66.25±18.19μm2,72.13±14.45μm2, 942.87±15.59μm2,1467.55±23.46μm2 and 3313.04±41.74μm2;The Wuzhishan pigs’ pancreas glucagon immune reaction cell distribution area were 227.91±7.51μm2, 271.94±14.76μm2,3624.02±28.91μm2,6531.8±48.98μm2 and 12 510.09±65.25μm2; The Wuzhishan pigs’ pancreas somatostatin immune reaction cell distribution area were 15.69±3.61μm2,21.58±2.13μm2,198.28±16.62μm2,384.53±35.46μm2 and 873± 25.02μm2oTo sum up, the post-embryonic Wuzhishan pig pancreas morphological characteristics changed with the changing day age.The composition of pancreatic islet cells number increased with the increasing day age. And the main component cells had differentiated and proliferation ability before the 6 months age. This study will provide morphology test data for Wuzhishan pig pancreas genetic research, diabetes and other diseases of pancreas.