| In this experiment, we studied genetic diversity from many aspects. A total of 134 wild soybean sample were collected from the border between Yanbian area and North Korea, which mainly concentrated in five areas. According to the geographical position, we divide all the wild soybean into five populations A, B, C, D, and E. Furthermore the representative materials, including Hailong, Huaide, Yitong, Changbai and Beijing.In order to research the genetic diversity and relationship in wild soybean. The 134 sample (population (134)), population A, population B, population C, population D and population E were analyzed by using 34 pair of SSR molecular markers with well reproducibility and polymorphism. The results are as follows:1. By using the 34 pairs of SSR molecular markers, we detect 317 allelic variants, on the average of 9.32. Shannon-weaver index distribution from 0.7727 to 2.3034. with a mean of 1.6707. Expected Heterozygosity index distribution from 0.3474 to 0.8346, with a mean of 0.74137. This results show that wild soybean population) 134) presents a highly genetic divergence in the border between China and North Korea.2. The index of subdivision (Fst) among the populations is 0.3633, and the gene flow (Nm) is 0.4635, these indicated that the populations had about 36.33%of genetic variations. It means higher frequency of gene flow among populations.3. The coefficient of genetic similarity is between 0.1558 and 0.5961,with an average of 0.3684. From the clustering results, there was significant correlation between population genetic similarity and geographical distance. |
