Establishment On Enterocoelia Animal Model Of Echinococcus Muhilocularis In Cricetulus Migratorius

To make animal model of Echinococcus muhilocularis in Cricetulus migratorius, and observe growthrhythm of E.m in enterocoelia of C.migratorius. Establish a simple and rapid observation animalmodels of hydatid disease with methods of naked eye observation and direct microscopicexamination. Detection of E.m antibodies from serum of Cricetulus migratorius。C. migratorius were vaccinated by enterocoelia at2000cephalomere of E.m dose each animal; Measureof the cystica weight and positive antibody, count on ratio of cystica weight/body weight, and observationof growth rhythm of E.m were did in C.migratorius at10,15,18,22,40,60,80,100d after infection.Growth of the E.m and infection rate of cystica were compared at100d after vaccinating at doses of100，500and2000to10of the animal separately.59of62C. migratorus were infected by E.m. Maturecephalomere of E.m at18d, which reached100%of infection rate, and positive antibody at15d wereobserved after infection. Ratio of cystica weight/body weight was over15%at60d.100%rates of theinfection were observed at100d after infected by the three doses. There have been characteristics for highinfected rate, short period of the infection, easy observation of growth of E.m and antibody titer on theestablishment of animal model of E. m in enterocoelia of C. migratorius. The60th day after infected E.mcan be used as observation period of making animal model in C. migratorius. It will be principal referencetargets of making animal model of E.m in the animal for ratio of cystica weight/body weight, antibody titerand level of cystica growth. The female is better than the male for making animal model for E.m in C.migratorius. Using methods of naked eye observation and direct microscopic examination to establish aInitial E.m Cricetulus migratorius animal models of hydatid disease which is a simple and rapidobservation method to qualitative and quantitative.