| These study were conducted to investigate the effect of tributyrin (TB) andcoated sodium butyrate (SB) on intestinal histomorphology，mucosal disaccharidaseactivity and lipid metabolism in lipopolysaccharide (LPS)-challenged broilers.One hundred sixty one-day-old Cobb broilers (45.1±0.5g) were randomlyassigned to4treatment groups: control group, LPS group, TB group and SB group.Each treatment comprised4replicates,10broilers per replicate. The control andLPS groups were fed with basal diet; the TB group was fed the basal dietsupplemented with500mg/kg tributyrin (45%); the SB group was fed the basal dietsupplemented with877mg/kg sodium butyrate (30%). Five broilers with averagebody weight of (1.5±0.2kg) were chosen from every replicate on d37of the age.On d38, d40, and d42of the age, broilers in LPS group, TB group and SB groupwere injected intraperitoneally with LPS (500μg/kg of BW), whereas the broilers inthe control group was injected with same volume of the sterile saline. Four hourspost the LPS or saline injection, blood samples were obtained from the wing vein.On d43of the age, the broilers were killed and tissue samples were collected.The results shows as follows:1. The effect of tributyrin and sodium butyrate on intestinal morphologicalstructure and mucosal disaccharidase activity in LPS-challenged broilers.(1) Administration of LPS decreased the mucosal protein content in theduodenum (P <0.05) and the DNA content in the jejunum (P <0.05), whereasincreased the TP/DNA ratio in jejunum (P <0.01). Diet supplementation of TBincreased the RNA content in duodenum(P <0.05) and diet of SB increased theDNA content in jejunum (P <0.01), dcreased TP/DNA ratio in jejunum (P <0.01).Compared with the control group, the TB group decreased the mucosa proteincontent in the duodenum (P <0.05), increased the TP/DNA ratio in jejunum(P <0.05). Diet summplemented with SB increased the DNA content in jejunum(P <0.05), but decreased the RNA/DNA ratio in duodenum (P <0.05) and the TP/DNA ratio in jejunum (P <0.01) in comparison with the control group.Additionally, compared with the TB group, SB group increased the DNA content injejunum (P <0.01) and decreased TP/DNA ratio in jejunum (P <0.01).(2) LPS injection decreased the villus height in duodenum (P <0.01) and jejunum(P <0.05), the ratio of villus height to crypt depth in duodenum (P <0.01), jejunum(P <0.05) and ileum (P <0.01), the villus width in duodenum (P <0.05) andjejunum (P <0.01), and the villus surface area in jejunum (P <0.01), whileincreased the crypt depth in duodenum (P <0.01), jejunum (P <0.05) and ileum(P <0.01). However, supplementation of TB increased villus height (P <0.01) andthe ratio of villus height to crypt depth (P <0.05) in ileum, the villus width injejunum and ileum (P <0.01), and the villus surface area in ileum (P <0.01), thecrypt depth in duodenum (P <0.05) and ileum (P <0.01). Similarly,supplementation of SB increased the villus height in duodenum (P <0.05), the ratioof villus height to crypt depth in ileum (P <0.05), the villus width in duodenum,jejunum (P <0.01), the villus surface area in duodenum and jejunum (P <0.01).Compared with the TB group, diet of SB group increased the villus width induodenum and jejunum (P <0.01) and the villus surface area in duodenum andjejunum (P <0.01), while decreased the crypt depth in duodenum (P <0.05), thevillus height, and villus width and villus surface area in ileum (P <0.01) in briolers.These results showed that dietary TB or SB can effectively alleviate the intestinalinjury induced by LPS.(3) LPS stimulation decreased the activity of sucrase in jejunum (P <0.05) andileum (P <0.01), and the activity of maltase in duodenum, jejunum and ileum(P <0.01). Supplementation of TB increased the activity of sucrase in jejunum andileum (P <0.01), the activity of maltase in duodenum (P <0.05), jejunum (P <0.01)and ileum (P <0.05), and the activity of isomaltase in ileum (P <0.05).Supplementation with SB increased the activity of sucrase in ileum (P <0.05), andthe activity of maltase in duodenum, jejunum and ileum (P <0.05). Compared withthe SB group, the TB group increased the activity of sucrase and maltase in jejunum (P <0.01), the activity of isomaltase in ileum (P <0.05). The results showed thatdiet supplemented with TB and SB can effectively restore the decrease ofdisaccharidase activity induced by LPS in intestinal mucosa by LPS, and theefficiency of TB was better than that of SB.2. The effect of tributyrin and sodium butyrate on lipid metabolism inLPS-challenged broilers.(1) LPS injection decreased the HDL-C (P <0.05) and TCHO (P <0.01) levels,but increased the TG level (P <0.01). However, supplementation of TB increasedthe HDL-C (P <0.01) and TCHO (P <0.01) levels, whereas decreased the LDL-C(P <0.05) and TG (P <0.01) levels. Supplementation of SB increased the TCHOlevel (P <0.01), and decreased the TG level (P <0.01). Compared with the TBgroup, the SB group decreased the HDL-C level (P <0.05) and tended to increasethe LDL-C level (P <0.1). These results suggest that diet supplementation of TBand SB can attenuate the negative effects induced by LPS on serum lipidmetabolism in broilers.(2) Treatment of LPS increased the intramuscular fat in leg (P <0.01) andtended to increase the intramuscular fat in breast (P <0.1). Supplementation withTB decreased the intramuscular fat in breast and leg (P <0.01). Supplementationwith SB decreased the abdominal fat (P <0.05), and the intramuscular fat in breast(P <0.05) and leg (P <0.01). Compared with the TB group, the SB group decreasedthe intramuscular fat in leg (P <0.01) and the abdominal fat. The results showedthat diet supplementation with TB and SB can alleviate the increase of fatdeposition by LPS stimulation in broilers.In conclusion, dietary TB and SB can improve the intestinal histomorphologyand serum lipid metabolism, increase the activity of mucosal disaccharidase, anddecrease the deposition of tissue fat in LPS-challenged broilers. Moreover, dietsupplemented with500mg/kg45%TB was more effective in improving the ilealmorphology and increasing the activity of mucosa disaccharidase than that ofdietary SB. |
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