The Effect Of Different Medicine Dispose With Cytokines On Deep Partial Thickness Burn Canine

In order to establish the mode of deep partial thickness burn, to study the dynamic change rule of deep partial thickness burn,we carried on a series researchs. We treated12experimental dogs with three temperature and four duration.The burn depth were measured with clinical symptoms and pathological sections. Second.20mongrel canine were selected and randomly divided into4groups,4range of3cm2area were taken to scald part which on the lumbosacral.Group Ⅰ were treatment with nothing and group Ⅱ, Ⅲ, IV with ofloxacin, erythrocin and dexamethasone acetate ointment respectively.Cures were carried once per4h,3times a day. Venous blood were collected before the experiment (0h) and6h,12h,24h,48h post-burn and then been analyzed.Local symptoms were observed3d,7d,10d and21d post-burn. Areas of the wound tissues were measured and the healing time were recorded. In experiment three, grouping was the same as the previous study. Sera samples (Oh,6h,12h,24h,48h) and cytokines IL-6、IL-8、IL-10、TNF-α (Oh,6h,12h,24h,48h) were detected with ELISA kits. Results of the first study showed that the skin turned into reddish white or reddish yellow, oedema, ulcer and the skin base became pale under the condition of99℃,23s.Microscopic examination showed sweat glands and hair follicles were damaged, and so were the deep dermals. These results indicated the succeed of burn mode establishment. In experiment two, the clinical symptom grades of group Ⅰ and Ⅱ were significantly higher than group Ⅲ and IV of15d post-burn (P<0.05or P<0.01). Group Ⅰ was significantly higher than group Ⅲ (P<0.05). Within21d after the burn, the average healing rate of group Ⅲ and Ⅳ showed significant higher than group Ⅰ (P<0.05or P<0.01). WBC and GRA raise significantly in24h (P<0.01), MID raise significantly in12h (P<0.05), LYM reduced significantly (P<0.05). WBC and GRA of the treated groups were significantly lower than group Ⅰ within12h (P<0.05). After24h, erythrocin and dexamethasone groups showed significant higher than group Ⅰ in WBC and GRA levels (P<0.05). LYM of erythrocin and dexamethasone groups raise significantly in12h (P<0.05) and MID raised significantly in24h (P<0.01).With the results of the last study, IL-6and IL-8from sera and tissue raise significantly in24h (P<0.05), TNF-a raise significantly in12h (P<0.05), IL-10reduced extreme significantly in48h (P<0.01). IL-6、IL-8、IL-10、TNF-α of ofloxacin group kept non-significant to group I at any time points (P>0.05). IL-8from sera of erythrocin group was significantly lower than group I in24h (P<0.05) and IL-6、IL-8、IL-10、TNF-α from tissues were significantly lower or extrem significantly lower (P<0.05or P<0.01). IL-6、IL-8、IL-10、TNF-α of dexamethasone groups kept significantly lower or extrem significantly lower than group I at any time points (P<0.05or P<0.01) until24h(P>0.05).The conclusions of the three studies are listed below. Experiment one,99℃,23s can cause deep partial thickness burn by32layers pledget attach onto the lumbosacral.Exeperiment two, erythrocin can remit clinical symptoms with anti-inflammation in ealy stage of burn and suppress infection with antibacterial ability. Dexamethasone can control inflammation in early stage and evidently promote healing of the wound tissue. Ofloxacin can promote healing with antibacterial property and sterilization in the late stage. In the beginning phase of deep partial thickness burn, WBC, GRA and MID rise and LYM reduces. Right use of ofloxacin can reduce WBC and GRA in the early stage. The use of erythrocin in the early stage can control general inflammation effectively and then strengthen the phagocytosis and immunity. Dexamethasone can control inflammation in the early stage but may have some opposite effect in the late stage. Experiment three, pathological lesions of deep partial thickness burn can caused by over secretion of IL-6、IL-8、TNF-α and reduced expression of IL-10. Ofloxacin cannot regulate inflammatory cytokines obviously after deep partial thickness burn. Erythrocin and dexamethasone can both suppress the secretion of IL-6, IL-8and TNF-α, and slow down the reduction of IL-10.