Effects Of Four Plant Extracts On Methane Emission, Nutrients Metabolism And Rumen Microflora In Mutton Sheep

This article aimed to study the effect of mulberry leaf flavonoids, resveratrol, allicin and tea saponinon methane emission, nutrients metabolism and rumen fermentation and microorganism.The concreteresearch content was divided into the following3tests.Experiment1: Screening of mulberry leaf flavonoids, resveratrol, allicin and tea saponin optimumaddition levels on methane emissions in mutton sheepThis study was conducted to investigate the screening of mulberry leaf flavonoids, resveratrol, allicinand tea saponin optimum addition levels on methane emissions in mutton sheep.Fifteen healthy dorper (♂)×thin-tailed han (♀) crossbred ewes (F1) with average body weight of60.0±1.73kg were selected. AddedMLF,Res,AL and TS, which supplementation levels were0.25,0.5,1.0,2.0, and4.0g/d for MLF,0.125,0.25,0.5,1.0, and2.0g/d for Res,0.5,1.0,1.5,2.0, and2.5g/d for AL and1.6,2.0, and2.4g/d for TS.The experiment was divided into1control and4test periods, using self-controlled trial design, Feedingbasal diet added different plant extracts at the same time.Open-cycle Sable gas metabolism system wasused for gas metabolism test. The results showed that supplementation of2.0g MLF (P＞0.05),0.25g Res(P＜0.05),2.0g AL (P＞0.05) and2.0g TS (P＜0.05) significantly decreased6.38%,10.11%,6.38%and9.57%methane emission calculated based on metabolic units per kilogram of body weight in dry matterintake (L/kgW0.75kg-1·DMI), respectively, compared with the control group. Each of MLF, Res,AL and TScan reduce the CO2and methane emission and the optimum supplementation level should be2.0g/d,0.25g/d,2.0g/d and2.0g/d respectively.Experiment2: Effects of mulberry leaf flavonoids, resveratrol, allicin and tea saponin on methaneemission and digestion metabolism in mutton sheepThis study was conducted to investigate the effect of dietary supplementation of mulberry leafflavonoids, resveratrol, allicin and tea saponin on methane emission and digestion metabolism on muttonsheep, with an aim to provide reference for reducing methane and improving energy efficiency. Ten healthydorper (♂)×thin-tailed han (♀) crossbred ewes(F1) with average body weight of60.0±1.73kg wereselected, the experiment was divided into1control and4test period, using self-controlled trial design,feeding basal diet added different plant extracts at the same time. The results showed that supplementationof MLF, Res, AL and TS could reduce methane emission calculated based on metabolic units per kilogramof body weight in dry matter intake(L/kgW0.75kg-1·DMI)and CO2output, and the addition of Res and TShad significant difference(P＜0.05).MLF could reduce apparent digestibility of DM, NDF and ADF and increase apparent digestibility of OM and N,but the difference was not significant(P＞0.05). Res, AL andTS significantly improved apparent digestibility of DM、OM、NDF、ADF and N(P＜0.05). MLF and Resreduced Nitrogen deposition rate(P＞0.05). AL and TS significantly increased Nitrogen deposition rate(P＜0.05). Add MLF, Res, AL or TS are obviously improved nutrient digestibility and metabolizable energy(P＜0.05) and reduced methane energy(P＞0.05). MLF, Res, AL and TS reduced methane emissions to someextent, and to improve the digestibility of nutrients, improved utilization of nutrients and energy.Experiment3: Effects of mulberry leaf flavonoids, resveratrol, allicin and tea Ssaponin on rumenfermentation and microrganismThis study was conducted to investigate the effect of dietary supplementation of mulberry leafflavonoids, resveratrol, allicin and tea saponin on rumen fermentation and mirooganism. Fifteen healthywith average body weight of70.0±1.78kg and install permanent rumen fistula adult wethers were selected.The experiment was divided into1control and4test periods, using single factor design. Feeding basal dietadded different plant extracts at the same time, and the positive test period was divided into five time pointsto collect rumen fluid for analysis. The results showed that, rumen pH values fluctuated between5.74~6.24with MLF, Res, AL or TS(P＞0.05). MLF and Res increased NH3-N(P＞0.05), AL and TS reducedNH3-N and TS was significant(P＜0.05). MLF,Res,AL and TS increased TVFA, propionic acid molepercent, butyrate, isobutyric,valeric and isovaleric(P＞0.05), decreased acetic acid mole percent and acetic/propionic value(P＞0.05). MLF, Res, AL and TS increased total bacteria, Fibrobacter succinogenes,Ruminococcus flaefaciens, Ruminococcus albus and Butyrivibrio fibrisolvens(P＞0.05) and decreasedMethanogens and Protozoan(P＞0.05). MLF, Res, AL and TS can affect rumen fermentation parametersand reducing Methanogens and Protozoan and increasing cellulose-decomposing bacteria which conduciveto the rumen fermentation.