Studies On Fauna Of Digenetic Trematode Of Fish And Phylogenetic Systematics Of Allocreadiidae In Irtysh River

Digenetic trematode were investigated from the Fishes in Irtysh River （in Chinese territory）.from theperiod of April2009to August2012.By using the conventional survey methods of fish parasitology andmolecular biology techniques. epidemiological characteristics and phylogenetic relationships were studiedin this paper, The main conclusions are as follows:（1）6species of Digenetic trematode were collected: including Allocreadium isoporum Siusarski,Allocreadium montanum Sidorov et Butenko, Allocreadium elongatum Achmerov., Carassotremakoreanum Park, Rhipidocotyle Diesing, Digeneans sp..which belonging to3Genus,3Family,2Orders.（2）From the results of Epidemiological investigations of Digenetic trematode, we found the infectedFishes Species were as follows:Esox lucius （Linnaeus）、Abramis brama orientalis （Berg）、Leuciscusleuciscus baicalensis （Dybowski）、 Leuciscus idus （Linnaeus）、 Gobio gobio acutipinnatus（Men’schikov）、Carassius auratus gibelio（Bloch） Perca fluviatilis （Linnaeus） with no seasonaldynamic variation. The infection can be Observed yearly with a peak in summer, while Occasionally occurin other seasons.whereas some Features about the Age of the host emerged: The infected fishes alwayswere2years old,obviously in Esox lucius （Linnaeus）and Leuciscus idus （Linnaeus）. EpidemiologicalAnalysis provides basic data for the prevention and control of Digenetic trematode of fishes.（3）The Allocreadium isoporum Siusarski were identified by Combined morphological and molecularbiology methods. ITS2rDNA sequences were Determined, Sequence alignment of the same family speciesby BLAST in NCBI, the results shows that the Homology are97.8%、98%、99.5%. then Phylogenetic treeestablished by Mega5.2neighbor-joining method.Different genera of Allocreadiidae clusteringrespectively,while3samples in this study Clustering with Allocreadium isoporum with highlyaffinity.Paracreptotrematina and Polylekithum were closer to outgroup means older position in the ancientevolutionary. Allocreadium Exactly Counter with this. Thus, Clarify the phylogenetic relationships withinthe Allocreadiidae preliminary.