To Detect The Dung E.coli O157:H7and Toxicity Studies In Different Regions Of Xinjiang

E. coli O157causes hemorrhagic diarrhea and enteritis, O157:H7serotype as representative strains. Todetect the dung E. coli O157:H7in Ili, Tacheng and Akesu of Xinjiang, Understand the prevalence about E.coli O157:H7in different cattles of Xinjiang. After EC broth enrichment and immunomagnetic beadsaccumulation, SMAC plate and MUG broth were used to select the bacteria, rfbe and fliC gene of selectedbacteria were test by PCR, and biochemical test methods was taken for compliance inspection. Detect the10strains of E coli O157:H7from319cattle in the Xinjiang, the detection rate was3.11%. This studyresults show that the part of the cattle camp exist threat possibility by E. coli O157:H7. This research use Oantigen gene, flagella H antigen gene, intimin and Shiga toxins as specific genes for amplification. Selected5primers fragments of different size, After a series of trial and error parameters of the reaction the reactiontemperature and time. Through observation and detection of clinical response, mortality rate and bloodabout mice infected with E. coli O157:H7, Analysis different pathogenic mechanism of pathogenicvirulence factor and about the pathogen carried. the experiment in mice (♀) poison attack experiment wascarried out after weighing, grouping, continuous record against poison mice after clinical symptoms and thetime of death, all the control mice tail vein blood after4hours, making blood smear at the same time, allthe whole blood samples for final routine blood test. Mice infected with E. coli O157:H7depressed of spirit,abdominal pain, unresponsive and other symptoms, with few mild diarrhea, urine clear yellow. Pathologicautopsy visible small parts congestion, severe bleeding, intestinal contents have colorless transparentmucus. The blood routine detection with E. coli O157:H7SLT number of white blood cells in mice, thetrend of granulocyte count were significantly decreased, and the blank control group mice extremelysignificant difference (P<0.01); Not with E. coli O157:H7SLT number of lymphocytes in micesignificantly difference compared with the blank control group (P﹤0.05), with E. coli O157:H7SLT micelymphocyte count extremely significant difference compared with the blank control group mice (P﹤0.01);With E. coli O157:H7SLT with mice with E. coli O157:H7SLT there are differences between red bloodvolume in mice, significant difference (P﹤0.05).