| Strawberry continuous cropping obstacles seriously restrict the current level ofstrawberry production, so strawberry production is in urgent need of varieties ofenvironmentally-friendly and effective continuous cropping obstacles to realize thesustainable development of strawberry production. In this paper, the strawberry resistanceto continuous cropping obstacles mutant Tz-125as a test material, the mass production ofpropagation process was optimized in technology; during the mutant taking-root stage, andacclimatization stage, exogenous hormone Methyl Jasmonate, MeJA was applied to themin order to study the influence of physiological and disease resistance caused by this kindof plant hormones on mutant plants, and soil pot experiment to explore Methyl Jasmonate,Methyl Jasmonate on strawberry mutant resistance to the effect of continuous croppingobstacles through the continuous cropping; in this experiment the mutant got vaccinatedwith Glomus mosseae in the acclimatization stage and observed the physical growth oftransplanting continuous cropping soil to potted plants to research the effect of Mosesballoon mold on the strawberry mutant Tz-125of continuous cropping obstacle resistanceof strawberry. The main results were as follows:1. Based on the MS culture medium added hormones ratio was0.1mg/L, Indolebutyric acid+0.2mg/L, Naphthalene acetic acid+1mg/L. With6-benzyl amino purinedealing with it, the multiple shoot clumps multiplication factor was the largest, reaching6.7, which was the optimal choice of strawberry mutant Tz-125multiple shoot clumpsinduced proliferation. Based on the MS culture medium, the root system showed theaverage root number was large, taproot stout, and root system developed; besides, theheight of seedling plants was moderate and seedling plants robust, when indolebutyric acidconcentration was0.5mg/L, which was the best use of concentration for root seedling.Nutritional soil: Vermiculite: the pearlite proportion for0:3:1. With half strength hoaglandnutrient solution of acclimatization the survival rate of it was the highest; in the20days itssurvival rate reached83.33%. The test showed the optimal proportion of seedling substrateof strawberry mutant Tz-125. 2. Methyl jasmonic acid process of strawberry mutant Tz-125root showed obviouslow to promote high inhibitory effect when Methyl jasmonic acid concentration was2×10-7mg/L,which showed the optimal condition of seedling plants taking roots, the formation ofroot early time and high rooting rate.3. In the acclimatization stage, after methyl jasmonic acid treatment could promotevegetative growth of strawberry mutant Tz-125but differences between differentconcentration process were not obvious. At the same time Methyl jasmonic acid treatmentcould significantly improve strawberry seedlings strains of superoxide dismutase (SOD)and phenylalanine ammonia enzyme activity and kept a higher level of activity in a longtime so as to improve seedling plant disease resistance. By processing of jasmonic acidmethyl ester, to a certain extent, the seedling plants within a much longer time could keepthe content of malondialdehyde in a low in order to improve the resistance of the mutantplants, and achieve the effect of disease resistance.4. In the pot experiment of continuous cropping soil, when applied methyl jasmonicacid concentration was2×10-7mg/L—the treatment could strengthen the strawberry mutantTz-125resistance and its effect of continuous cropping obstacle was the best. To28daysthe two groups of treatment of seedlings were in plant nutrition, seedling survival rate was100%, and non-inductive disease.5. Moses balloon mold in the acclimatization stage of the strawberry mutant Tz-125successfully infected seedling root and parasitic and formed a clump planting and vesiclestructure. After continuous cropping soil pot experiment, it proved that Moses balloonmold could improve the survival rate of strawberry plants and its vegetative growth levelof strawberry plants, thus strengthening the mutant resistant to strawberry continuouscropping obstacle. |
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