| In order to study the effects of different energy and protein levels, flax-seed andvitamin E on performance and beef quality in Holstein fattening bulls, this study made twoparts experiments.Experiment1: Effects of different energy and protein levels in diets on growthperformance and beef quality of3-12month-old Holstein bulls403-month-old Holstein bulls with the weight of98kg were randomly divided intofour groups, each group with10calves. The experiment was divided into five stages. Theexperiment diet included four protein levels and two energy levels. The results showed that:with the increasing of energy and protein levels, group â…¢ showed the best growth rate,which indicated9.84%(P<0.01) and7.14%(P<0.05) higher than group â… and group â…¡,respectively. The serum aspartate aminotransferase of group â…£increased17.56%(P<0.05)than group â… , the serum growth hormone of group â…¢ and â…£increased48.78%(P<0.05)and42.68%(P<0.05)than group â… , respectively; the apparentdigestibility of crude protein in group â…¢ was3.92%(P<0.05) higher than that of groupâ… a nd2.85%(P<0.05) higher than that of group â…¡, respectively; with increasing ofenergy and protein levels, the PUFA concentration of longissimus muscle improved, groupâ…£ increased6.04%(P<0.05)than group â… . Compared with group â… , group â…¡ and groupâ…£, the economic benefits ofgroup â…¢was higher1.64Yuan/(d·heads),1.23Yuan/(d·heads) and0.62Yuan/(d·heads), respectively. Therefore, using Holstein bulls produceveal, nutrient levels for each stages suggested were as follows:13.63MJ/kg digestibleenergy,20.00%crude protein of100~150kg stages;13.26MJ/kg digestible energy,18.50%crude protein of150~200kg;13.26MJ/kg digestible energy,17.50%crudeprotein of200~250kg;13.35MJ/kg digestible energy,16.50%crude protein of250~300kg;13.72MJ/kg digestible energy,14.50%crude protein of300~350kg.Experiment2: Effects of Flax-seed and Vitamin E on performance and beef quality inHolstein fattening bulls75BW520kg of Holstein bulls were randomly divided into five groups, each groupwith15bulls, there were no obvious differences in initial weight between the fivegroups(P>0.05). Group â… was the control group, feeding based dietï¼›group â…¡with10% Flax-seed instead a part of the concentrate, group Ⅲ~Ⅴadding Vitamin E with200mg/kg,400mg/kg and600mg/kg, as the basis of group â…¡. The results showed that:adding10%Flax-seed, the average daily gain increased significantly; the F/G wasdecreased, shear force, cooking loss, and the antioxidant ability of muscle were decreasedsignificantly; when adding Vitamin E, the antioxidant ability was increased. Comparedwith the control group, the diet adding10%Flax-seed(group Ⅱ~Ⅴ), the percentage ofPUFA increased. The n-3PUFA of group Ⅱ~Ⅴ increasedby24.59%(P<0.01),28.42%(P<0.01),42.62%(P<0.01),39.89%(P<0.01). The n-6/n-3of group â…¡, â…¢, â…£,â…¤increased18.41%(P<0.01),20.33%(P<0.01),24.18%(P<0.01) and23.08%(P<0.01)thangroup â… . Based on the analysis of all indicators, when using10%flaxseed to replace partof the diet to fatten Holstein bulls, can be used with Vitamin E. The appropriate level ofVitamin E was400mg/kg to600mg/kg(DM basis). |