The Study Of Monitoring And Diagnostic Methods Of Metritis In Dairy Cows At Different Postparturient Stages

Object: The object of the research was to study on the diagnosis and treatment methods withendometritis in the early postpartum cows, to reduce the economic losses in dairy farms.Methods: In this research, B ultrasound technique was applied to the diagnosis of cow’s uterusdisease based on history investigation, observation of clinical symptoms and rectal examination.Sonographies for endometritis, pyometra, uterus effusion and chronic endometritis mucosalhyperplasia were described and analysed. These studies provided the foundation forthe later experiments. The follow-up experiments were carried out in center dairy farm of WesternAnimal husbandry and in dairy farm of Western Junggar animal husbandry. The twenty two cowswere selected from each of both farms respectively. The cervix diameter and wall thickness ofuterine horn of these dairy cattles were determined in the early stages in postpartum (1-35days ofcalving) with application of B-ultrasound scanner to check the tracking (once every2days beforepostpartum11days and once every5days latter) two indexes.At the same time, blood sampleswere collected (1-35days of calving) when B-ultrasonography examination was applied in thetracking period. Concentrations of E2, P4, FSH, IL-8and TNF-a were detected by ELISA and thedata were statistically analyzed. In the end,32heads of postpartum repeat breeder dairy cattlewere selected as the experimental animals. B-ultrasound scanning method of uterine filling liquid,examination method of uterine reflux and urine were used to diagnose whether the these dairycows were subclinical endometritis. The dairy cattles of subclinical endometritis were treated withOxytetracycline injection solution and the new Chlorhexidine acetate suspension.Results: The results showed the382cows with clinical uterine disease were scanned byB-ultrasound and established the typical sonographic atlas of clinical metritis. The results ofmonitored dairy cows in the early postpartum stage showed that there were not significantdifference in the firstborn cows (P>0.05) between the normal and the metritis groups on twoindexes of the longitudinal diameters of cervix and the thickness of the uterine horns walls. In themultiparity cows, the longitudinal diameter of cervix between the normal and the metritis were notsignificant difference as well, however, there is significant difference between the two groups thatthe wall thickness of the uterine horns in the first seven days postpartum (P＜0.01), but were notsignificant difference in the subsequent stages (P>0.05). The concentrations of serum E2betweennormal and metritis cows were significant difference in the9th and35th day in the postpartumperiods (P <0.05), but were not significant in the other stages (P>0.05). The serum concentrationsP4between normal and metritis cows were significant difference in the27th day in the postpartumperiods (P<0.05), but were not significant in the other stages (P>0.05). The concentrations ofserum FSH between normal and metritis cows were not significant in the various postpartumstages cows (P>0.05). The concentrations of IL-8and TNF-a in the serum between normal andmetritis cows were not significant in the early postpartum (1-35days in the postpartum) stages (P>0.05). Infertility cows of suspected subclinical endometritis were diagnosed with the twomethods combined the B-ultrasound inspection in the uterine filling liquid with determination ofthe uterus return liquid. The results indicated that detection rate of the above method were higherand more accurate than diagnosis of reagent methods. There were not significant difference(P>0.05)both Oxytetracycline and new Chlorhexidine acetate suspension on therapeutic effect.Conclusion: In the conclusion, B-ultrasound technique could be used as assisting means ofdiagnostic the cows with clinical uterine diseases. The changes of the longitudinal diameters ofcervix, the thickness of the uterine horns walls and the serum concentrations of E2, P4, FSH, IL-8and TNF-a in different stages of early postpartum cows could be used as reference indexes in theprocess of the uterine infection monitoring and diagnosis. The method combine the B-ultrasoundinspection in the uterine filling liquid with determination of the uterus return liquid could be usedto diagnose subclinical endometritis more accurately.