The Research Of OB Gene Polymorphism And The Association Between OB Gene And Growth Traits

In order to promote the work of molecular marker assisted selection, the test researched the polymorphism of ob gene and the association between ob gene and the weight. The mutation of C-T was detected in exon 3 of the ob gene of Tan sheep by the technique of sequencing and PCR-RFLP, mutation homozygote was not discovered. Chi-square test showed that the frequency of AB and BB was in Hardy-Weinbery equilibrium. The effective genotype was AB, of which the frequency was 0.05. The result of correlation analysis was that the effects of the mutation on three-month weight and six-month weight were significant (P<0.05).The hypothesis testing of interaction effect display that the interaction between genotype and gender was not significant (P>0.05).The result provides reference resources for determining a breeding marker of Tan sheep growth traits.