| Black currant (Ribe. Nigrum L) is an ancient representative species of small berries, which has a high value of nutrition and health. It is widely cultivated in our country but with serious secondary germination phenomena in the cultivation process of some areas,that is in need to be resolved as soon as possible. In order to find the respiratory metabolism secondary germination, provide a theoretical basis for solving related to the further study of the phenomenon of black currant secondary germination.In this study, Adrian varieties was selected as materials, labor regulation by exogenous hormone GA and ABA mainly, the respiratory rate, respiratory pathway (EMP, TCA, PPP), respiratory substrate, and control of breathing ways and carbohydrates (respiratory substrate) key enzyme activities were measured and analyzed in the secondary germination process.The main findings are as follows:1 Treating with exogenous GA3 and ABA on Adrian, they had significant effect of regulation on the second germination compared with the control (water treatment). GA3 treatment have a strong role in promoting,while ABA treatment significantly inhibited.2 With different concentrations of GA3, the promoting effect was different in the black currant secondary germination, it was more obvious and the time of germination was shorter with the increase of concentration. Germination rates were not significantly different with three concentration of GA3, the final germination rates could reach more than 95%.3 Different concentrations of GA3 treatment, shoot length formed after germination, there are significant differences. The lower the concentration, the shorter the length of the branch germination, the shorter the inter-section, as the concentration is increased, the results showed the opposite. ABA significantly inhibited the second germination of black currant, treatment with 50 mg/L ABA, the germination rate was only about 3%, and the buds were small, about 80% of the control.4 After 30mg/L GA3 treatment, bud total respiratory rate were significantly higher, and 50mg/L ABA treatment, the difference was significant. For respiratory route concerned, GA? significantly enhanced TCA way rate at around 10d peaked for the control of 1.652 times, the difference was significant (P<0.01); PPP approach across the secondary during germination changed significantly, very significantly higher than the ABA treatment and water control; EMP pathway respiration rate showed "down-up-down" trend.5 three respiratory pathway key enzyme activity and respiration respiratory pathway has a similar process of change, but much earlier respiration pathway respiration rate. And key enzyme activity in GA3 treatment was significantly higher than under the control and ABA treatment, and shoot under ABA treatment activity was significantly lower than the control and GA3.6 Black currant secondary germination process there are multiple electron transport pathway, after GA3 treatment, pre-cyanide-resistant pathway was activated, and the increase was significantly higher than the ABA treatment, while the control group was significantly higher than GA3 and ABA treatment (P<0.01). But GA3 treatment, cytochrome pathway respiration 44% higher than the control, significant differences, is the second major electronics germination process pipeline. In contrast, the lower inner bud ABA treatment cytochrome pathway respiration run activity, there is a continuous fluctuations but volatility is relatively small, the test is only slightly higher than the pre-control value, the latter as a whole were lower than the control group and the control group between significantly different (P<0.01).7 Respiratory substrate plays an important role in respiratory metabolism. In the secondary germination of black currant, a rapid increase of soluble sugar, the latter declined slightly; Starch content showed a downward trend, a drop of 29.73%. the presence of sucrose is always the most basic form of sugar in the bud, also contains fructose, glucose and xylose. With the early germination, sucrose content had a downward trend, of fructose and glucose content were increased rapidly. SPS as sucrose metabolism enzyme activity was "V" type curve, maximum 130.7 umolg-FMh-1. SS inverted "bimodal" curve and a maximum 140 umolg-1FMh-18 We had clearly seen in the total respiration bud significantly enhanced by GA3 treatment, respiration pathways changed significantly that provide a wealth of metabolic energy for germination. In contrast, ABA treatment reduced total respiration within the bud, the energy that provided by respiration was reducing, presuming lack of energy may be main reason of ABA inhibited secondary germination of black currant. |
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