The Study Of Creating Multi-resistance And Long Shelf Life Tomato Materials By Marker-assisted Selection

Tomatoes are one of the world’s most cost-effective vegetables, it is also a vegetable which can infection a lot of diseases. Tomato disease has been a major obstacle to high quality and high yield. Most pesticides are useless to the diseases and will cause environmental pollution. Breeding resistant varieties is the most economical and effective measure. Tomatoes are easily damaged during transportation and storage and it seriously affected their economic benefits. So, it is necessary for the market to breed multi-resistant and long shelf-life varieties.With the help of pyramiding technology, we hybridize different tomato materials with different resistance genes and storage gene. Then we screen their F2 and F3 populations by molecular marker assistant selection in order to elect multi-resistant and long shelf-life materials. What we done lay a solid foundation for breeding multi-resistant、high yield and quality tomato varieties. The results were as follows:1. We use the female parent of “Moonlight” tomato, 13485(with Cf-5、Tm-1 and Mi-1),cross with male parent 13065(with Ty-2 、Ty-3、I-2、Ph-2 and Ph-3) and 13337(with rin) respectively, make the hybrid: 13485×13065, 13485×13337, and build F1 and F2 populations.2. We got 2~4 candidate markers that can separately identify Cf-5, Mi, Ty-2, Ty-3, I-2, Ph-2, Ph-3, and rin gene by review many literatures. Utilize male and female parents、F1 and other materials to test the accuracy of the markers. Chose the markers that can totally match all the materials and have good stability to screen the F2 populations.3. In order to improve the efficiency of the molecular marker-assisted selection, we employed three co-dominant markers, T0302-1, P6-25 and Z1063, that tightly linked to tomato yellow leaf curl virus disease-resistant genes Ty-2, Ty-3 and tomato Fusarium wilt resistant gene I-2, respectively, and we established a multi-PCR technique that can identify these genes simultaneously using the three makers.4. Using selected markers to screen the F2 populations of 13485x13065 and 13485x13337.We got 19 materials can resistant to 5 diseases and 30 materials can resistant to 4 diseases from the F2 populations of 13485x13065. We also got 20 materials with rin and can resistant to 3 diseases. Survey horticulture traits of the 69 materials, chose 26 into the next generation.5. Select 200 materials of the 13485x13065 F2 generation to inoculate Fulvia and 200 materials to inoculate Fusarium; And also, select 100 materials of the 13485x13337 F2 generation to inoculate root-knot nematodes and 100 materials to inoculate tobacco mosaic virus. Verify the consistency of MAS and vaccination.6. Build 26 F3 populations and identify their genotype. Planting those which have more homozygous genes. Keep doing this until they got finally homozygous.