Formulate At Least Three Kinds Of Resistant Germplasm In Tomato

Tomato is a kind of cosmopolitan vegetables with high yield, nutrient-rich and good taste, andthis tomato can be used like fruit. Tomato disease, however, is a big factor to limit the productionof tomato, and now the dangers of tomato disease and pest become so serious that cast a shadow onthe expansion of tomato cultivation area. At present, breeding0f cultivars resistant to many kindsof diseases of tomato is the most effective way to control plant diseases and pests. Usingconventional breeding methods to cultivate the multi-resistant tomato cultivars need a long timeand heavy workload. In addition, these conventional breeding methods are highly and easilyaffected by the environment and geography. But the molecular marker technology combined withtraditional breeding method to cultivate germplasm resources of tomato which are resistant to avariety of diseases, not only can guarantee the accuracy of the target traits of plant, but also canreduce the workload and shorten the breeding period.In this study, by using Multiple Parents Technology, I hybridized the tomato leaf mould,tomato toot knot nematodes, tomato fusarium wilt and tomato yellow leaf curl virus diseases intoone material. I fostered a good quality tomato which is resistant to a variety of diseases using themethod which combined artificial pathogen identification with marker-assisted selection, andestablished the foundation of the use of the gene pyramiding breeding of fine tomato varieties. Themain results are as follows:1. I get five kinds of triple hybrids F1generation which contain anti-tomato leaf mold (Cf-5),anti-tomato root knot nematode (Mi), anti-tomato wilt (I-2) and resistance to tomato yellow leafcurl virus disease (Ty-1, Ty-2).I used multiple parents in this experiment and self-pollination of thematerial. In this study I also got triple hybrids of F2and F3generations of tomato plants.2. I selected the tomato resistant primer from a large number of related literature. I fond fourpairs of specific primers after calculating and comparing specific primers for△G values, Tmvalues and CG content, as well as the inspection of single primer PCR specific amplification. Thisfour pairs of specific primers can be used in multiplex PCR.3. This experiment introduces Multiple-PCR technique, which can amplify the Cf-5、Mi、Ty-1and Ty-2genes at the same time by screening the specificity of the primers, and prove the accuracyand applicability of the technique through repeating the experiment. The technology can screen the molecular markers of the tomato leaf mildew, the tomato root knot nematode and the gene relatedtomato yellow leaf virus disease. And combinedg with traditional breeding methods, thistechnology can also rapidly select out the tomato germplasm resources of excellent quality andresistant to these three diseases.4. With the Multiple-PCR technique, I eliminated the false hybrid strains which could notresist more than three kinds of diseases and rejected the strains with weak growth status by usingthe molecular marker-assisted selection of the selfing generations of the triple hybrid in this study.Then I kept the plants which showed the result of DNA specific markers coincided with the resultof artificial inoculation identification after I identified the rest of the plants by identification ofartificial induction. Eventually I screened out two strains containing the Cf-5, Mi and Ty-13typesof resistant genes, whitch showed a high resistance to the tomato leaf mildew, the tomato root knotnematode and the tomato yellow leaf virus disease. This excellent germplasm resource has manyadvantages, such as strong stress resistance, high productivity and good palatability.