Cloning, Expression And Function Analysis Of CmERFⅠ-7 And CmERFⅢ-1 Gene In Cucumis Melo

Melon is a widely cultivated and significant commercial value horticultural crops all over the world, Melon has become an important model plant of fleshy fruit about molecular mechanisms in the field of fruit maturation. ERF subfamily are compents of the ethylene singal transduction pathway, which participate in stress signaling pathways cross and play a role in the signal crossing pathway on connection. In this study, look limacteric ripening fruit of melon as research subjects, We successfully cloned a cDNA fragment of CmERFⅠ-7 and CmERFⅢ-1. The cDNA fragment length of CmERFⅠ-7 and CmERFⅢ-1 were 761 bp and 748 bp respectively. Quantitative real-time PCR analysis showed that the CmERFI-7 and CmERFⅢ-1 gene were expressed in different development periods and different organs of melon. With analysis of different tissues, CmERFⅠ-7 had the highest expression in the ovary, and CmERFⅢ-1 had the highest expression in the leaf. At different developmental stages of fruit, the highest expression of CmERFⅠ-7 appeared in 10 days after pollination, and the highest expression of CmERFⅢ-1 appeared in 20 days after pollination. By constructing RNAi expression vector and overexpression vector, which were infected melon mesocarp through agrobacterium of transient expression technology. Finally, we detected transient expression of the sample with Real-time PCR. The results showed that the injection site emerged yellow phenotype by overexpression vector, and the injection site delayed yellowing by RNAi vectors. The results of real-time quantitative PCR showed that CmERFⅠ-7 and CmERFⅢ-1 gene mRNA expression increased significantly after overexpression compared with the control; CmERFⅠ-7 and CmERFⅢ-1 gene mRNA expression compared with the control also varying degrees of rise after interference.