| In this study, five-month-old dairy goats were Yanbian arginine, reserpine and other hormone treatment, the5-month-old Yanbian normal lactating dairy goats before getting a lactation period, and5-month-old dairy goat Yanbian secretion of milk were milk quality analysis showed that after induction of exogenous hormones and nutrients through adult Yanbian milk dairy goat colostrum secretion of pregnancy compared to protein, lactose and other dairy goat Yanbian5months produced no significant differences.After the use of exogenous hormones secrete milk processing Yanbian5-month-old dairy goat, on its side injected into the breast tissue of about100μg of plasmid pBC-NEO-HSA by ELSA testing,6h secretion of milk in the beginning found expression the purpose of the gene, at about10h to reach the highest expression reached86.7μg/L,17h express stops. Description5-month-old dairy goat mammary Yanbian successfully detected the expression of target genes.In this study, the conclusions are:1.5-month-old dairy goat Yanbian after hormone treatment can produce milk.2. By comparison, arginine do better suited than reserpine-induced lactation dairy goats drugs.3.5months exogenous hormones to induce the secretion of milk goats Yanbian first day-the first seven days after Yanbian milk compared with the same period of pregnancy dairy goat milk secretion, the main nutrient content was not significant.4Exogenous hormones to induce the secretion of milk goats Yanbian5months old the first eight days-the first28days after Yanbian milk compared with the same period of pregnancy dairy goat milk secretion, the main difference between the content of nutrients significantly.5. The mammary gland-specific expression vector pBC-NEO-HSA was injected into the breast milk of goats, the success of target gene expression was detected, indicating that the carrier can be constructed recombinant HSA synthesis in dairy goat mammary gland... |
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