| Buckwheat is rich in a multitude of nutrients including flavonoids(mainly rutin), dietary fiber and various vitamins, especially medicinal composition. It also contains some special trace elements and, is currently regarded as one of the healthy food resources in 21 st century.. Buckwheat soba is one of the major two Fagopyrum cultivars, whose growth period is short but demonstrates great tolerance and adaptability to adversity like drought and infertility while its utilization rate of light and heat is effective as well. In China, even buckwheat just remains a kind of minor grain crop, however its position in serving as the food safety guarantee in arid and semi-arid areas is of paramount importance.Buckwheat self-incompatibility and cross-pollination characteristics have led to its low maturing rate, which is an important factor affecting its production. Self-incompatibility, as a prevalent phenomenon presents in many plants such as, cabbage, rape and cabbage and other crops. Self-incompatibility studies have showed that due to the S-locus genes, the recognition or non-recognition between pollen and stigma has resulted in the abnormal germination of pollen upon stigma, or the restriction of the prolonging of pollen tube in the column or the inhibition of the fertilizing process, whose consequence can be the loss of fructification. Buckwheat has the same type of flowers and shaped flowers, the same type of flower is common in wild species. Buckwheat flowers can be divided into two types in accordance with its shape, long style and short style, Studies have already confirmed that both the self- and cross-pollinations of buckwheat cannot fruit, in other words, they are self-incompatibility.only the combination of different types can produce seeds, suggesting that hybridization involved in selfing or between the same type flowers is determined to be incompatible.This research has performed the the common buckwheat hybridization between different flowers with the same type, aiming to observe the styles of hybrids, sturdy and agronomic traits; Through the molecular marker conducted on the mRNA produced within 1-1.5h right after the hybrid and pollination of different common buckwheat columns with distinct genetic background, a certain number of polymorphic primers were selected. Preliminary research showed:(1) The types of the columns of F1 and F2 generations which came from the hybridization between general flower type and the same column pattern of common buckwheat have presented different separation rates. It is manifested that the genetic regularity of column could be complicated, however, according to the F2 Chi-square test in combination 1, the segregation ratio of column fits the three genotypes mode.(2) common buckwheat style type may be divided into five categories, including long styles, short styles, long slightly styles, short slightly styles and complete style. It is not sure in long slightly styles,short slightly stylesand complete style of the three types regarding their fruiting rate(3) There are 24 pairs of buckwheat SSR primers showing a good amplification effect in the leaf DNA of the different type flowers(long and short columns) and the same column plant in the overall 32 primers used in this study. Particularly, 6 primers have exhibited an abundant polymorphism in the amplification of the type,they are GB-FE-014, BM85, BM87, BM84, BM071, 1094 respectively. In addition, BM024 and BM047 have been verified to be relevant with the genetic background of common buckwheat whereas BM85 is efficient for the identification of long, short and same column types buckwheat coming from different genetic sources.(4) Last but not least, 16 pairs of primers have revealed the capacity to amplify the differential bands in the mRNA of the progeny of two different type flowers(long and short columns) in terms of their selfing and cross-hybridization. Among them, 3 primers displayed a good polymorphism, they are GB-FE-110ã€BM071 and BM062 seperately. |
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