| In order to improve the development of synchronicity of Brassica oleracea L. single bud microspore, and perfect the system of the isolated microspore culture technology, this experiment through artificial pruning, spraying NAA and increasing micronutrient fertilizer on the donor plant of microspore to preliminary research on factors that affect the bud microspore development of synchronicity. The results show that only keep the primary lateral branch(CL2) or treated by 1000mg/L(N2) both can improve the bud microspore development of synchronicity effectively. After increased 0.1% micronutrient fertilizer, the biggest ratio of pull over mononuclear period up to 76.0% that significantly improved than in contrast which is 35.6%.1. In the artificial pruning process, and at the same growth period and bud length. The ratio of pull over mononuclear period of three kinds training is significantly improved than contrast at 4.0 mm length. The treatment named CL1, CL2 and CL3 improved 141.4%, 149.0% and 84.0% respectively compared with the contrast. And the maximum ratio of the three types of processing appears at 4.2mm length increased by 36.1%, 39.9% and 36.1% respectively than the contrast. There, the CL2 is the ideal way of training which can effectively improve the synchronicity of development of microspore, in addition, with a lot of buds.2. In the same bud section, the bud length of bud is longer than the contrast with the increase of nutrition level, and the flowering time is also different. Especially the result of CL1, its flowering phase of CL1 extended 2 to 3 weeks than the contrast.3. In the results with different concentrations of NAA treatment, when the length of buds are the same, The microspore ratio of pull over mononuclear period of the treatment of N2 and N3 are all significantly higher than contrast which length is at 3.5mm and 4.2mm. The microspore ratios of pull over mononuclear period of the three treatments have a increase which is 16.0%-238.4% compared with the control at the bud length of 3.5mm and 4.2mm. In a word, treated by 1000mg/L(N2) both can improve the bud microspore development of synchronicity effectively.4. After increasing micronutrient fertilizer can significantly improve the microspore development of synchronicity. When the buds lengths of the control and treatment are the same between 4.5mm to 5.2mm. The microspore ratio of pull over mononuclear period of the treatment is 1.1%-76.0% that is significantly higher than contrast which ratio scope is 8.6%-35.6%.5. In the increasing micronutrient process. At the beginning of flowering, the higher ratio of pull over mononuclear period of treatment that is 29.4%-49.3% which flower bud node section is 13-15 upwards from the flowering bud,it has a significantly improve than contrast that the higher ratio range from 19.6% to 32.3% which flower bud node section is 12-14 upwards from the flowering bud. There, the suitable bud length range is the same which is 5.0-5.5 mm. At the full-blossom period, the bud node section of higher ratios of both the control and treatment are 12 to 14 and 13 to 15 respectively which the bud lengths respectively both are 4.5-5.1mm. The ratios of its corresponding range are 9.7% to 25.6% and 26.9% to 49.2% respectively, and the ratio of treatment significantly increased compared with the contrast. As a result, increasing micronutrient can improve the microspore development of synchronicity effectively to some extent. |
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