The Effect Of Vascular Endothelial Growth Factor On Progesterone Production And Proliferation Of Cultured Immortalized Luteal Cells Of Goats

To investigate the effect of VEGF(Vascular Endothelial Growth Factor) and VEGFR-2(Vascular Endothelial Growth Factor receptor 2) on Co-culture cells(Luteal cells and Endothelial cells) proliferation and progesterone production.Immortalized luteal cells and endothelial cells were co-cultured by cell culture; MTT assay was used to detect the cell proliferation; ELISA was applied to observe the P4 concentration and the relative expression of steroid enzyme were tested by RT-PCR.The results were described as follows:1. VEGF of different concentration could improve cell differentiation significantly both at 48 hrs and 72 hrs, especially when the concentration of VEGF were 10 ng/ml and 100ng/ml.2. VEGF of different concentration could improve P4 production significantly both at 48 hrs and 72 hrs, but there was no effect at 24 hrs of VEGF.3. SU1498 only had no effect on cell proliferation, but it acted on the co-culture cells when together with VEGF. We observed that the cell proliferation was decreased with SU1498+VEGF(10ng/ml), however, it would increase when the concentration of VEGF enhanced to 100ng/ml. Both of the effect were detected at 72 hrs.4. SU1498 inhibited the synthesis of P4 both at 48 and 72 hrs, as well as SU+VEGF group, whatever the concentration of VEGF were. VEGF could not help to improve the effect of SU1498 on P4 secretion.To sum up: the results indicated a dose-and-time dependent effect of VEGF on cell proliferation and P4 production, and there was also a saturability of VEGF.SU1498, a potent inhibitor of VEGFR-2, could decrease P4 secretion effectively. SU1498 had chemotaxis effect on cell proliferation with the stimulation of VEGF.