| Cibotium barometz, belonging to the Dicksoniaceae family, is widely distributed in China. The rhizomes is perennial recovery medicine, has the functions of dispelling wind dampness, nourishing liver and kidney, strengthening waist and knee functions, suitable for backache, wind cold dampness arthralgia, deadlimb, hemiplegia and other symptoms. Cibotium barometz as landscape plant, is popular with elegant figure and beautiful gesture. As a result of Cibotium barometz officinal value and economic value, its wild resources are excessive excavation. At the same time, owing to relative lag of artificial propagation and cultivation, the species resources dropped year after year. As key protection plant of National level Ⅱ and restrictions on the export species, the introduction of protection and artificial cultivation are extremely urgent. Therefore, the influencing factors of spore germination, gametophyte development and seedling development were studied by three ways of sterile breeding, soil propagation and division propagation, in order to provide theoretical basis for improvement of Cibotium barometz spore propagation system and later large scale production. Experiments and results as follows:(1) Setting different storage temperature (-20℃>4℃ and room temperature) and different storage time (200d、250d、300d、350d and 400d) to explore the influencing factors of Cibotium barometz spore germination. The results showed that the germination rate of Cibotium barometz was lower at the storage temperature of-20℃ and room temperature (20-25℃); the germination rate was higher at the storage temperature of 4℃. With the increasing of storage time, spore germination rate had increasing trend; the germination rate of Cibotium barometz was the highest, when spore storage time is 350 d. Therefore, putting Cibotium barometz spore storage at 4℃ was beneficial to germplasm conservation; and seeding Cibotium barometz spore after storage about one year was beneficial to spore germination.(2) Using Orthogonal Design, the effects of different concentrations of inorganic salt, sucrose, GA3,6-BA and IAA on the spore germination were studied. The results showed that the optimum concentration of inorganic salt、sucrose、 GA3,6-BA and IAA were different during a series of process of spore germination、 gametophyte development and sporophyte development. The optimum-medium of Cibotium barometz spore germination is:1/8MS+cane sugar at 30g/L+0.04mg/L 6-BA+0.02mg/L IAA. The best medium of transforming gametophyte into sporophyte is:1/2MS+cane sugar at 30g/L+0.04mg/L GA3+0.02mg/L 6-BA.(3) To explore the best condition of Cibotium barometz spore germination, gametophyte development and seedling development were studied in a split plot experiment with 2 culture conditions,3 substrate ratio and 3 sowing density by routine propagation:The mixed soil (the nutrient soil, muck and sand blend in the volume ratio of 1:1:1) was the optimal growing medium for spore routine propagation; seeding density of 2mg per nursing media was beneficial to transform gametophyte into sporophyte.(4) Single-factor experiment was designed to investigate the influential factor-transplanting time of the Cibotium barometz sporophyte growth and later development, The results showed that the suitable transplanting time of Cibotium barometz seedling was after the third sporophyll appeared. Cibotium barometz seedling, well-growing, had a higher survival rate.(5) The optimum period and ramet specification of Cibotium barometz division propagation were studied. The results showed that, in early March, the seedling rate of Cibotium barometz ramets reached 83.33%. The appropriate ramet size was benefit for forming seedling. The seedling rate was higher when the rhizome length was 21-30 cm or rhizome width was 3-6 cm. Therefore, early sowing and suitable ramet size were benefit for Cibotium barometz seedling formation. |
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