| In our research, a multiple PCR amplification system was established to detectcytoplasmic genetic diversities of potato breeding resources, and this system wasverified by analyzing cytoplasmic genetic backgrounds of331breeding materialsbelonging to five populations including a diploid inbred line, AF line originatedfrom a single hybrid strain, FM line selected from multiple hybrid strains, populationS and common cultivated varieties in China. These results can be used as referencesto identify, collect and develop and utilize potato germplasms. The main results are:(1) A multiple PCR amplification system was established to detect types ofcytoplasm by4chloroplast DNA markers (T, S, SAC and A) and one mitochondriaDNA marker (D) simultaneously. We optimized PCR system of these reported fivepairs of DNA markers, including primers mixing ratio, annealing temperature anddosage of templates DNA. This multiple PCR system is an effective way forlarge-scale analysis the cytoplasm genetic background of potato breeding resourcesrapidly to select crossing parents and early generation effectively.(2) The cytoplasmic genetic diversity of331potato breeding materials wasanalyzed by the established multiple PCR system and mitochondria DNA markerof cob/rps10locus. The cytoplasm of all of the testing materials can be divided intoT, D, A and W four types, accounted for20.2%,30.5%,0.9%and48.3%respectively;the W cytoplasm can be divided into T/β, A/β, W/α[D], W/α, W/β and W/γ. The ratioof T/β (typical in S. tuberosum ssp. tuberosum), W/α[D](common in hexaploid S.demissum), any other wild types and A/β (familiar in S. tuberosum ssp. andigenum)were20.2%,30.5%,48.4%and0.9%, respectively. The results indicated that thecytoplasmic genetic backgrounds of all testing materials were rich in wild typecytoplasm and can be utilized to widen the genetic backgrounds of commoncultivars. |
