| Anthurium andraeanum (Araceae) is one of the most popular flowers in the world and has a larger planting area in south China. In recent years, stem basal rot disease was severe and had a great influence on its production. Pathogen identification of the diease and fungicide screening for the disease control, were carried out in this thesis. The results showed that.1. The disease samples were collected from Conghua Production base of the Flowers Research Center of Guangzhou in three batches from May to August2012.52isolates of the pathogens from the disease were obtained. Among them,9isolates belonged to Pythium,8were Rhizoctonia, and35were Fusarium. The result of pathogenicity test showed that3isolates, which belonged to Pythium, Rhizoctonia and Fusarium respectively, could cause the disease via alone infection or complex infection. The result of the pathogen identification through morphological and molecular methods indicated that3isolates of the pathogen were Pythium splendens, Rhizoctonia solani and Fusarium oxysporum, respectively.2. The result of inhibition test of11fungicides, i.e. Kresoxim-methyl pyrazole, prochloraz, ymexazol, benomyl, metalaxyl, aluminum phosphine, propamocarb, fludioxonil, azoxystrobin, double propargyl amine acid and jinggangmycin, to three pathegens, showed that precision-fludioxonil and metalaxyl had excellent inhibition effect on the mycellium of Pythium splendens, which EC50was less than0.15μg/mL; precision-fludioxonil, kresoxim-methyl pyrazole and benomyl opposing had excellent effect on Rhizoctonia solani, which EC50was less than0.6μg/mL; prochloraz and refined-fludioxonil had significant effect on Fusarium oxysporum, which EC50were0.2μg/ml and0.78μg/ml, respectively.3. The inhibition test of Paenibacillus polymyma SHL-1against mycelium growth of the pathogens showed that SHL-1had good inhibition effect on Rhizoctonia solani and Fusarium oxysporum, but had no inhibition effect on Pythium splendens. |
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