The Effect Of Progesterone On The Molity Of Rabbit Duodenal Regulated By Autonomic Nerver

In order to explore the effects of progesterone on the motility of rabbit duodenalregulated autonomic nerve, we used the immunohistochemistry SP method to detect thedistribution of progesterone receptor(PR) in female rabbits duodenum as well as theacquisition and processing system of biomedical signal to observe the combined effects ofprogesterone and norepinephrine as well as chloride acetylcholine on the amplitude andfrequency of duodenal contraction.The test results are obtained as follows:1.Immunohistochemical SP method showed that the progesterone receptors were strongpositive in the duodenal smooth muscle and serosa of the rabbit, while weak in duodenal gland,intestinal villi, submucosa and other non-muscular structure. Image analysis showed PR in theduodenal smooth muscle and serosa was extremely significant compared with other non-muscle structures.2. Physiological experiments showed that the amplitude and frequency of duodenalcontraction was respectively declinede, declined and heightened with the increase ofconcentration of single role of progestin, norepinephrine and chloride acetylcholine.According to the concentration-amplitude curve of progesterone, norepinephrine andacetylcholine, the correlated function was y=0.1143x2-0.9547x+2.6414(R2=0.9896), y=0.0027x2-0.1545x+2.6742(R2=0.9899), y=-0.0207x2+0.3689x+2.4711(R2=0.9632)respectively. When observing the combined effects of progesterone and norepinephrine (orchloride acetylcholine) on the amplitude and frequency of duodenal contraction, aftercompletely blocking acetylcholine receptors, we found that in the concentration range of10-8g/ml-3.2x10-7g/ml,the drop of amplitude and frequency of duodenal contraction was observedwith the increase of norepinephrine, while progesterone can antagonize the inhibition ofnorepinephrine on the duodenum within the concentration range of0-3.2ug/mL,and theantagonism was enhanced with the increase of progesterone. When the concentration ofnorepinephrine increased to3.2x10-7g/ml, even if progesterone reached the maximumconcentration, its antagonism was not obvious. After completely blocking adrenergic receptor,we found that in the concentration range of10-8g/ml-8x10-8g/ml,the rise of amplitude and frequency of duodenal contraction was witnessed with the increase of chloride acetylcholine,while progesterone can antagonize the excitement of chloride acetylcholine on the duodenumwithin the concentration range of0-3.2ug/ml,and the antagonism was enhanced with theincrease of progesterone and more obvious due to the increase of chloride acetylcholine.These results show that the duodenal smooth muscle and serosa act as the target organsof progesterone, suggesting that progesterone may directly affect the gastrointestinal tract on thelevel of the effectors of visceral reflex arc, and the antagonistic effect of progesterone on theneurotransmitters suggest that progesterone may regulate gastrointestinal motility and play arole in the pregnant animals by affecting the activities of neurotransmitters...