Multifactorial Methods Construct Ascites Syndrome Of Broilers And Protective Effect Of Vascular Endothelial Function From Qi Ling Gui Fu Fang For The Disease

Ascites syndrome (ascites syndrome of broilers, ABS), also known as pulmonary hypertension syndrome (pulmonary hypertension syndrome, PHS) is a chronic broiler nutrition metabolic disease, ascites in2-3d will die, the incidence rate of over60%, the mortality rate as high as42%. Ascites experimental model building syndrome and effective prevention methods, effective drug screening for the disease and its significance. The research contents and results are as follows:A multi-factor approach to build ascites syndrome:This article uses multivariate model was constructed of the disease.1-day-old Ross156broilers at15days of age were randomly divided into three groups, the control group:50,(22±1.5℃) environment rearing, feeding conventional feed; hypothermia model group:53,(11±2℃) Environmental breeding, feeding conventional feed; multi-factor model group (M):53,(11±2℃) environment farming, dietary lard3%,4%fish meal, drinking water by Na+concentration was0.12%added NaCl. The results showed that:the average weight of45-day-old broilers,35, and45-day-old lung tissue and TNOS cNOS activity, low temperature model and multi-factor model of body weight was significantly lower than the control group (P<0.01). During the observation period the" incidence of ascites syndrome, ascites expression of cardiac index,35and45-day-old lung tissue iNOS activity, NO content, ET-1levels, ETAR mRNA and VEGF mRNA, and low-temperature model and multi-factor model group significantly higher than the control group (P<0.01). Conclusion:The method can be successfully constructed multivariate ascites syndrome model.Second, the ventral side of Qi Ling normalized endothelial function in broiler ascites syndrome:the15-day-old Ross broilers were divided into six groups, one control group (normal temperature (22±1.5℃), conventional feed); remaining five groups At low temperatures, high-energy and high-sodium diet feeding water to induce ascites syndrome, in which the model group does not give any medication, L-Arg group (1%L-Arg), high, medium and low dose group of traditional Chinese medicine (by broilers per kg of body weight administered2g,1g,0.5g). Decoction concentration1g/ml. The results showed that the average weight of45-day-old broilers,35, and45-day-old lung tissue and TNOS cNOS activity, the model group was significantly less than the control group (P<0.01). The incidence of ascites syndrome, the expression of cardiac ascites index,35and45-day-old lung tissue iNOS activity, NO content, ET-1levels, ETAR mRNA and VEGF mRNA in the model group was significantly higher than the control group during the observation period (P<0.01). High, medium and low dose group of Chinese medicine, L-Arg group compared with the model group, high dose group and the detection indicators L-Arg group have been significantly improved, the middle dose group also had some improvement, indicates that high doses of medicine has prevention and treatment of ascites syndrome effect. Conclusion:Fang Qi Ling go belly model for broiler ascites syndrome have a preventive effect.