Stem Tip Culture And Rapid Propagation Of Pinglu Edible Lily

In this experiment, the influence on induction of proliferation and differentiation of edible lily stem tip had been studied by different kinds and concentration of hormone, culture. The conditions temperature and light for the culture also bad been considered, and established edible lily rapid reproduction system.The main results were as followings:(1) The best explants disinfection method for edible lily is75%alcohol disinfection for30s, then the sterile water flush twice.10%sodium hypochlorite sterilization for12mins, sterile water flushed4-5times.(2) Through different hormone screening test, the optimal culture medium for the growth of the stem tip of edible lily was found out. The result was1/2ms+6-BA0.5mg/L+0.1mg/L NAA, induction rate could reach83.40%, it showed that Shoot tips grew fast and young plants were strong.(3) The optimal culture medium that induced young plants of edible lily produce bulbs was1/2MS+6-BA0.8mg/L+NAA0.1mg/L, the induction rate run up to80.67%, the induced Bulblets grew robust and strong.; Improveing sucrose concentration training to10%sucrose concentration could increase the induction rate to82.8%.(4) the optimal culture medium for Virus-free edible lily bulb multiplication culture in the MS medium was6-BA0.8mg/L and NAA、0.1mg/L, the proliferation and differentiation was multiple, Bulb proliferation rate reached7.3times. In terms of ZT, the ratio of NAA and IBA the optimal culture medium for virus-free edible lily bulb proliferation of cultivating was ZT2.5mg/L and NAA0.2mg/L, Proliferation rate of edible lily was6.8times, showed that the roots were robust, strong, green, grew faster and had many buds.(5) Additional6-BA0.5mg/L、NAA0.1mg/L in the1/2MS medium also can promote the proliferation of virus-free edible lily bulb, The average number of bulbs was7.7.(6)The optimal culture medium of edible lily scales to differentiate was6-BA1.5mg/L+0.1mg/L NAA, edible lily scales to differentiate was81.00%. The average number of each scale differentiation was8.3, showed that the roots were robust, strong, grew faster and had many buds; under the ratio of6-BA and IBA, the optimal culture medium of edible lily scales to differentiate was6-BA1.5mg/L+IBA0.1mg/L, edible lily scales to differentiate was66.00%, The average number of each scale differentiation was7.4, showed that the roots were robust, strong, grew faster and had many buds.(7) Vaccination position for differentiation of scales was that differentiation of the scales under scales section was greater than the differentiation of scales on scales section; the differentiation of the vertical insert was greater than the differentiation of scales in the flatwise orientation.(8) The optimal rooting medium for edible lily seedlings was1/2MS+NAAO.1mg/L, the rooting rate was as high as100%, the average root number was9.11, and root was stout and suitable for transplanting.The most suitable matrix for virus-free seed transplanting was1/2soil+1/2vermiculite, the highest survival rate reached93%.