Studies On Antiaging Effects And Mechanism Of Luteolin

Objectives:To investigate the antiaging effects of Luteolin on MRC-5cell lines and explore it’s antiaging mechanisms.Methods:3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test were used to determine maximum no-cytotoxic concentration on MRC-5cells. It’s antiaging activity was determined though Senescence-associated P-galactosidase Staining Assay, Measurement of intracellular reactive oxygen species and Lifespan Assay of C.Elegans. At last, western blot technical was used to detect the changes of key proteins on the AMPK signaling pathways and study the mechanism of antiaging of Luteolin.Results:1. SA-P-Gal staining assay show that the staining cells number were extremely decreased at the25μM of Luteolin. There were no apparent effects at the blow of25μM.2. Luteolin could extremely decrease intracellular reactive oxygen species levels,up to two times compared with model group at50μM. It also could notablely reduce ROS level at25μM.3. The lifespan assay of C.Elegans. showed that Luteolin could extend C. Elegans. Lifespan in a dose-dependent manner, and It could increase the lifespan up to one week at50μM. Therefore, it’s antiaging effects were confirmed.4. Western blot Analysis indicated that Luteolin up-regulated the expression of PGC-1α protein and phosphorylated AMPK protein and finally activated AMPK signaling pathways.Conclusions:Luteolin possessed antiaging activities and its mechanism may be that it can active AMPK/PGC-1α signaling pathway and regulate mitochondrial biogenesis.