Effect Of Dissolving Phosphorus Fungi On Soil Phosphorus Forms And Enzyme Activity And Rape Production In Reclamed Soil Of Mining Area

This paper described that65solubilizing phosphorus fungi were sperated from a total of220calcareous soil samples which were collected from18counties and8cities of Shanxi province.9of them were selected by dissolving phosphorus ability test of the strains. The observation of the fungi characteristics results that9strains were divided into three types. And ultimately3advantage strains of different characteristics were obtained through the observation.3advantage strains were identified by18sRNA sequencing as a result of Z40belongs to Mortierella,Z60belongs to Aspergillus niger and Z63belongs to Penicillium. In this paper, rape pot experiment studied solubilizing phosphate fungi application in mine reclamation. Conclusions are as follows:(1)The amount of dissoving tricalcium phosphate of Mortierella Z40and Aspergillus niger Z60and Penicillium Z63were387.51、684.37、459.19mg/L. Among them, Penicillium Z63have a strong ability to dissolve AIPO4and FePO4, the maximum amount of dissolved phosphorus respectively were649.74mg/L and221.38mg/L.Effect of different carbon and nitrogen sources on dissolving phosphorus of Penicillium Z63indicated that:the amount of dissolved phosphorus is maximum for702.59mg/L when carbon source was sucrose, nitrogen was nitrate. Medium of Penicillium Z63orthogonal test showed:the effect of dissolved phosphorus factors sorting was:carbon> pH> nitrogen> inoculum.(2) Pot experiment showed that:in different rape grown times,soil available P content in mine reclamation soil of different treatments were significantly higher than CK2and CK1. When rape was grown in mid time,soil available P reached the highest.And treatment Aspergillus niger+Penicillium was the most significant impact, acted as102.60mg/kg. (3) The measure of reclamed soil by calcareous soil classification method showed that:the content of Ca2-P in different solubilizing phosphate fungi treatments were significantly higher than the CK1and CK2, treatment Aspergillus niger+Penicillium was the highest, dissolution of Ca8-P, Ca10-P under different solubilizing phosphate treatments reached to a significant level, treatment Mortierella+Aspergillus niger+Penicillium dissolved the highest.(4) Applicating the Langmuir equation to fit the soil phosphorus adsorption in pot experiment, the correlation of different treatments all reached to a significant level. The maximum amount of P absorption under different solubilizing phosphate treatments was in526.32-625mg/kg, MBC was in22.5-24.38mg/kg, different solubilizing phosphate treatments were significantly reduced reclamad soil adsorption of phosphorus. Treatment Aspergillus niger did the most significant effect on reducing soil adsorption of P; soil average desorption rate was in21.14%-34.57%under different treatments. Amount of soil P desorption under Mortierella+Aspergillus niger+Penicillium was the highest in all different treatments.(5)The result of soil microbial biomass phosphorus showed that:soil microbial biomass phosphorus was increased significantly under treatments Aspergillus niger and Aspergillus niger+Penicillium and Mortierella+Aspergillus niger+Penicillium.lt increased138.17%and204.24%and127.70%than CK. The others were not remarkable.(6)Tthe solubilizing phosphorus fungi treatment Mortierella+Aspergillus niger had a superior effect on acid and alkaline phosphatase activity, respectively were31.51and21.10mg/100dry soil (37℃,24h),increased87.11%and117.38%than CK2.(7) Fresh weight and chlorophyll content of rape under different solubilizing phosphate treatments in the pot experiment reached a significant level than CK2, average of fresh weight and chlorophyll content of treatment Penicillium and Mortierella+Aspergillus niger+Penicillium were the most significant which reached240.67g/plant and1.08mg/g.