Research On The Effects Of Inoculation Mycorrhizal Fungus To Cultivate Artemisia And Artemisinin Content

This paper is based on the successful cultivation of Artemisia. Mycorrhizal fungi, which were extracted from Artemisia roots, were inoculated in the growth process of Artemisia, after separation, purification and identification, in order to observe and analysis the effects of mycorrhizal fungi on artemisinin content of Artemisia plants and its impact on the growth conditions. For analysis and discussion, inoculation method used in this research were mainly single bacterial strain inoculation and composite strain inoculation. The main experimental results of this experiment are as follows:Four types of mycorrhizal fungi were extracted from Artemisia roots and used CTAB method for biological identification. After DNA extraction, PCR, universal DNA Purification Kit, connection transformation and other steps, DNA fragments of the four columns of mycorrhizal fungi were acquired. By sequence alignment, the four types of mycorrhizal fungi were confirmed as Fusarium solani, Fusarium oxysporum, Penicillium pinophilum, and Fusarium proliferatum, using A, B, C, and D to represent them respectively.Artemisia seeds were sown in paper tube. After Artemisia seedlings, researcher removed weeds and too closed Artemisia seedlings in seedling pots to leavie every seedling in a single hole. After Artemisia seedlings grew strong enough, transplanted them to farmland.After2months of growth, researcher inoculated A, B. C and D mycorrhiza fungi to Artemisia roots separately, then orthogonally inoculated. After4months of growth, Artemisia plants of each group were measured for biomass on the same day. The results displayed that the separately vaccination of four mycorrhiza fungi to Artemisia promoted the crown biomass, stem height, number of lateral branches, etc. of Artemisia to some extent; mycorrhiza fungi C had no effect in promoting stem base top of Artemisia.After orthogonally inoculating mycorrhiza fungi, the best combination of Artemisia stem height was:inoculated mycorrhiza fungi A solution for80ml, mycorrhiza fungi B solution for70ml. mycorrhiza fungi C solution for70ml, and mycorrhiza fungi D solution for80ml. The best combination of Artemisia crown biomass was:inoculated mycorrhiza fungi A solution for90ml. mycorrhiza fungi B solution for90ml, mycorrhiza fungi C solution for70ml, and mycorrhiza fungi D solution for90ml. The best combination of Artemisia stem base top was:inoculated mycorrhiza fungi A solution for80ml, mycorrhiza fungi B solution for70ml, mycorrhiza fungi C solution for70ml. and mycorrhiza fungi D solution for80ml. The best combination of the number of Artemisia lateral branches was:inoculated mycorrhiza fungi A solution for80ml. mycorrhiza fungi B solution for70ml, mycorrhiza fungi C solution for70ml, and mycorrhiza fungi D solution for80ml.The respective determination of artemisinin content in Artemisia which were inoculated A. B, C and D mycorrhiza fungi separately showed that:mycorrhiza fungi D(Fusarium proliferatum) had a lowering effect on Artemisia artemisinin content, while the other three had a promoting effect. Orthogonal complex vaccinated the other three mycorrhiza fungi which had a promoting effect on artemisinin content to Artemisia, and determinated the Artemisia’s artemisinin content, researchers found that the best combination was mycorrhiza fungi A solution60ml, mycorrhiza fungi B solution80ml, and mycorrhiza fungi C solution80ml. Orthogonal complex vaccinated the four mycorrhiza fungi to Artemisia and determinated the Artemisia’s artemisinin content, researchers found that the best combination was mycorrhiza fungi A solution60ml, mycorrhiza fungi B solution70ml, and mycorrhiza fungi B solution80ml. In the end, the research also determined the infection rate of these four mycorrhiza fungi.