Combined Effect Of Molybdenum And Cadmium On Liver Function And MRNA Expression Of Ceruloplasmin And Metallothionein1in Duck

This experiment was conducted to observe combined effect of molybdenum andcadmium on liver function and expression of liver CP and MT-1mRNA in duck. In thisexperiment, ammonium heptamolybdate [(NH4)6Mo7O24·4H2O] and cadmium sulfate(3CdSO4.8H2O) had been used as the source of molybdenum and cadmium.360(180+180) healthy one-day-old Jiangnan2sheldrake were divided randomly into six groups:control group, low molybdenum group, high molybdenum group, cadmium group, lowmolybdenum cadmium group and high molybdenum cadmium group.The control groupwas fed basal diet. The experimental group was fed basal diet with Mo15mg/kg,Mo100mg/kg,Cd4mg/kg,Mo15mg/kg+Cd4mg/kg and Mo100mg/kg+Cd4mg/kgseparately. The experimental period was120days. Ten ducks of each group were chosen atrandom to collect related samples.The samples were used to observe pathological changeof liver, determine liver function and expression of liver CP and MT-1mRNA.The resultsare as follows:1. Compared with control group, liver index in experimental groups were increasedsignificantly（P<0.05）,the serum of ALT, AST, GLB, TBIL, ALP, LDH in experimentalgroups were increased significantly （P<0.05）; serum ALB,GLU decreased significantly（P<0.05）, moreover, tendency in groups with Mo-Cd concentration are obvious thansimple molybdenum group or simple cadmium group.2. The hepatocyte showed different degrees of granular degeneration, vesiculardegeneration and fatty degeneration. Mo and Cd had a dose and time-dependent.3. Compared with control group, the mRNA expression of liver CP in experimentalgroups were increased significantly（P<0.05）on the120th day; the mRNA expression ofliver MT-1were increased significantly（P<0.05）in Cd group and low Mo-Cd group.The results showed that different levels of molybdenum and cadmium could cause thechange of liver function, hepatocyte degeneration, and increase the mRNA expression ofliver CP, this indicated that Mo had a synergistic effect with Cd; Mo and Cd had anantagonistic effect on mRNA expression of liver MT-1.