Research On Effects Of Restorer Genes Rf3、Rf4from Guangxi Common Wild Rice (Oryza Rufipogon Griff.) And Mapping Of Rf3

The seed setting in hybrid rice greatly contributes to high and stable grain yield, which is determined by the fertility restorer genes in restorer lines. Thus, the selection and application of restorer lines plays an important role in raising rice yield. As the ancestor of Asia cultivated rice (Oryza sativa L.), common wild rice(Oryza rufipogon Griff) possesses many elite genes like fertility restorer. Previous studies showed that there was a high level of genetic diversity in restoring loci Rf3and Rf4in common wild rice of Guangxi, which provided the basis to broaden the new source for hybrid rice restoring genes.In this study, single segment substitution lines (SSSLs) were developed from two crosses between two Guangxi common wild rice, DP32and DP70, and9311, respectively. Each line possessed Rf3or Rf4loci, respectively. To investigate the restoring ability of Rf3and Rf4in these SSSLs, they were crossed to a typical WA-CMS lines,"Mengkang A", and a new type FA-CMS line,"Jinnongl A", respectively. On this basis, we further mapped the fertility restorer gene Rf3. The results were as follows:1. Five SSSLs with Rf3or Rf4locus derived from the wild rice were developed, among them, two contained Rf3locus and three contained Rf4locus.2. The SSSL with Rf3locus from DP32could restore the fertility of Mengkang A, but that with Rf3locus from DP70could not.3. The Rf4locus either from DP32or DP7.0showed stronger restoring ability of fertility to WA-CMS than the Rf3locus.4. All SSSLs could not restore the fertility of Jinnongl A.5. Rf3gene was mapped within an interval of555.5kb between two SSR markers, RM1195and RM10338.The results of this study would facilitate the exploitation and utilization of restorer resources from the Guangxi common wild rice and provide the ideal materials for further fine mapping of Rf3and Rf4.