The Killing Effect Research Of Different Irradiation Dosage On Ascosphaera Apis

Apiculture is an important integral part of China’s agricultural production, but the growth of the bee has been the harm of various pathogenic microorganisms, especially the bee chalkbrood disease. Bee chalkbrood disease is caused by Ascosphaera apis fungus infection and fungal infections, bee larvae death started outbreak in China in the early1990s, the later, for beekeeping production in China has caused severe damage. But very unfortunately, so far, still no effective prevention and control methods. Study found that market pollen often contains Ascosphaera apis, apiary, after to buy such as bee pollen feed them susceptible to disease, with60Co irradiation kill bacteria in bee pollen Ascosphaera apis is the effective measure to cut off the chalk disease transmission, and the irradiation dose is not clear, lead to irradiation effect is uneven. This study Ascosphaera apis as the research object, explore different irradiation dose on the Ascosphaera apis killing effect, in order to clear the lowest effectively kill bees balloon irradiation dose, for60Co irradiation bees feed (especially the bee pollen) at the time of irradiation dose selection provides the basis.First of all from using bacteria-purification technology obtained Ascosphaera apis from cretaceous disease (Bee chalkbrood diseases) apis mellifera larvae, and combined with morphology, lactic acid phenol medan dyeing and5.8S rDNA sequence analysis technology for identification. At the same time, we preparated different concentration gradient of A. apis spore suspension, and added them to3d larvae feed to determine the median lethal dose, using the technique of artificial feeding bee larvae, statistical larvae prevalence, calculated by coach type bee balloon bacterium median lethal concentration of Apis mellifera larvae. Other, in order to determine the minimum radiation concentration, we used different doses to irradiate the feed which adding median lethal concentration of spores, and then through these feed to infecte larvae. Finally confirmed that makes the median lethal concentration Ascosphaera apis spores feed the minimum effective irradiation dose.All these results indicate:from the Apis mellifera larvae of bee chalkbrood disease bee in the body, colony morphology are consistent with the literature reports, Microscope the sporocyst diameter, spore length-width ratio of ball diameter and spore morphology such as same as the relevant reports or near. Molecular identificated the purification of bacteria Ascosphaera apis, amplified fragment size in accordance with the theoretical value, the result blast contrast with GenBank released Ascosphaera apis specific fragment sequence, contact ratio99%. Under the condition of captivity, the median lethal concentration of bacteria Ascosphaera apis on larvae was5.6×104/ml. After60Co to add the bee that makes the median lethal concentration balloon fungus spores after irradiation and feeding larvae under the condition of7.0kGy irradiation dose, larvae prevalence and the irradiation group had significant difference (P<0.05).