Effect Of Selenium Deficiency On Lipid Metabolism And Selenoproteins Expression In Adipose Tissue Of Chicken

Selenoprotein is the one of existing forms of Selenium (Se) in vivo. Se is an essential trace element for human and animal. Selenium deficiency can lead to abnormal body function, Such as white muscle disease, pancreatic atrophy, exudative diathesis and so on. Selenium deficiency can affect the expression of selenoproteins in tissue, also closely related to chronic inflammatory reaction. However, there has not been reported that the effects of Se on expression of selenoproteins, inflammation, and lipid metabolism in adipose tissue of chicken. In this experiment, laying hens was the research object, and120one-day-old chicks were randomly divided into two groups:Control group (C group) and Low-Se group (L group). And Control group (C group) was fed a conventional dietary which Se content was0.15mg/kg, Low-Se group (L group) was fed a low selenium dietary, Se content0.033mg/kg, and two groups have a same conventional feed. The chicken were killed by35,45,55d, respectively, and collected the adipose tissue of subcutaneous, visceraland and articular three parts in chicken. We detected the gene expression levels (included25Selenoproteins:GPX1, GPX2, GPX3, GPX4, DIO1, DIO2, DIO3, Txnrd1, Txnrd2, Txnrd3, SelW, Sepnl, Seppl, SelO, Selk, SelS, SelT, Sepxl,15-Sep, Seli, SelU, SelH, SelM, Selpb, SPS2; lipid metabolic related genes:PPARy, Adiponectin and Adiponectin receptor1and2, LPL, ACC and FASN and inflammatory factor genes:PGES, COX2, iNOS, TNF-α and NF-κB; heat shock protein genes:HSP27, HSP40, HSP60, HSP70, HSP90and GRP78) in chickens by RT-PCR to explore the effects of selenium deficiency on selenoproteins expression and lipid metabolism in adipose tissue in chicken.The results show that:1. In selenium deficiency chickens, the expression levels of gene related lipid metabolism PPARy, LPL, ACC and FASN mRNA were significantly increased, the expression levels of adiponectin and its receptor gene mRNA were significantly decreased in chicken adipose tissue, which, in Low-Se group, PPARy, adiponectin and its receptors, FASN and ACC gene expression quantity difference in3kinds of adipose tissue, LPL expression in visceral adipose tissue was significantly lower than that in subcutaneous and articular adipose tissue. These indicates that the effect of selenium deficiency on the lipid metabolism in visceral adipose tissue is less than subcutaneous and articular adipose tissue and selenium deficiency reduced the lipd metabolis in body adipose tissue.2. In selenium deficiency chickens, the expression levels of25kinds of selenoproteins mRNA in chicken adipose tissue were significantly reduced, wherein, the most significant differences of selenoprotein GPX1was in the articular adipose tissue, and the lowest in visceral adipose tissue, suggest that the effects of selenium deficiency on selenoprotein expression in articular adipose tissue was highest, selenium deficiency could reduce the expression of selenoprotein synthesis in adipose tissue and influence the functions in body.3. In selenium deficiency chickens, inflammatory cytokines PGE synthase, COX2, NF-kB, TNF-a, iNOS mRNA expression in chicken adipose tissue were significantly increased, suggested that the expression of these genes may be involved in selenium-induced adipose tissue inflammation process in chicken. Wherein, expression levels of PGE synthase, NF-kB, TNF-awere little difference in three kinds of adipose tissue, but inflammatory cytokines COX2and iNOS gene expression levels were lowest in visceral adipose tissue, suggesting selenium deficiency has a little effect on inflammation in visceral adipose tissue.4. In selenium deficiency chickens, HSP27, HSP40, HSP60, HSP70, HSP90and GRP78mRNA expression levels were significantly elevated in chicken adiose tissue, which, expression level of HSP70in visceral adipose tissue was the lowest, levels in subcutaneous adipose tissue was the highest, suggesting through HSPs way the body could resist damage of chicken adipose tissue caused by selenium deficiency, damage that selenium deficiency caused in visceral adipose tissue was minimal and max in subcutaneous adipose tissue.